Disinfection aseptic processes

Instruction on the basic principles of microbiology, microbial physiology, disinfection and sanitation, media selection and preparation, taxonomy, and sterilization as required by the nature of personnel involvement in aseptic processing... 

There are items that must be transferred into the aseptic processing area that cannot be treated within a sterilizer/oven. These include portable tanks, electronic equipment, and containers of sterile materials (ready-to-use items, sterile powders, environmental monitoring media, etc.). Air locks, pass-throughs, and similar designs are employed in which the exterior surfaces of the items are disinfected. The disinfection process may be completed by personnel outside and/or inside the aseptic area depending upon the specifics of the design. 

When equipment maintenance is carried out within the dean or aseptic area, dean instruments and tools are used, and the area is cleaned and disinfected before processing recommences if the required standards of deanliness and/or asepsis have not been maintained during the maintenance work. 

As far as possible equipment should be designed and installed so that maintenance and repairs may be carried out without additional personnel having to enter the clean aseptic areas. If maintenance must be carried out within these areas,personnel concerned should receive appropriate training in the elements of microbiology. When within the areas they should be appropriately dressed, and use tools and equipment which have been sterilized as far as possible. Areas entered for maintenance should be cleaned and disinfected before processing recommences if the required standards of cleanliness and/or aseptic have not been maintained during the work. 

For Aseptic Processing where disinfection is employed to further reduce the surface contamination level, the choice of disinfectants and the way that they are used should be described in a procedure, in addition, detergents, disinfectants and antiseptics should be supplied sterile, or be sterile-filtered or otherwise sterilised at the use-dilution, or be sterilised as a concentrate and diluted only with sterile water. Diluted disinfectants or antiseptics should not be stored. Containers should not be topped up. Disinfectants/detergents used should be validated and approved. When disinfectants are used, more than one type should be employed. Monitoring should be undertaken regularly to detect the development of resistant stains. Disinfectants and detergents used in Class 1 and 2 areas should be sterilised prior to use. 

Risk mitigating in pharmacies (disinfection at aseptic processes) can be attained by ... 

Disinfectants and Cleaning Processes Disinfectants are an integral part of cleaning processes for aseptic manufacture. They are usually purchased as con> centrated stock solutions for use they should be diluted in water of Purified Water or Water for Injection quality. They should be filtered into white areas, usually, because of their viscosity, through 0.4S pm sterilizing filters. Each diluted batch should be allocated a shelf-life, and different disinfectants should be used in documented rotation. 

Non-steriie products should not be processed in the same area and at the same time as sterile products. If non-steriie procedures are carried out in rooms normally designated as aseptic, these rooms must be suitably disinfected and flushed with filtered air. 

Clean, aseptic and other related processing areas should be cleaned frequently and thoroughly in accordance with a written program approved by the Quality Control Department. Where disinfectants are used, different types should be employed in rotation to discourage the development of resistant strains of micro-organisms. 

In its core interventions for BSI reduction, the CDC advocates patient education. Main components are (1) hand hygiene and basic infection control practices during catheter accessing process (e.g., aseptic technique, masks, hub disinfection), (2) access care at home, (3) role of and risks associated with catheters, and (4) knowing signs and symptoms of infection and when to notify the physician. Despite mixed results in other studies, Cheng et al. [10] described a 40% lower risk of hospitalization due to infection and a 51% mortality reduction in patients with chronic kidney disease who received a multidisciplinary education prior to dialysis onset. We conclude that patient education is a valuable tool in the prevention of CLABSIs. 

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