3- -2,5-diphenyl tetrazolium bromide

Dye oxidation (e.g., tetrazolium reductase activity with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, MTT 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt, WST-1 3- (4,5 -carboxymethoxyphenyl) -2-(4-sulfophenyl)-2 H-tetra-zolium, MTS 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, XTT 2,2 -di-p-nitrophenyl-5,5 -diphenyl-3,3 -(3,3 -dimethoxy-4,4 -diphe-nylenej-ditetrazolium chloride, NET), Alamar blue assays, ATP concentration (e.g., luciferase assay), oxygen consumption (e.g., oxygen electrodes, phosphorescent oxygen-sensitive dyes), mitochondrial protein and nucleic acid synthesis mitochondrial mass (e.g., mitotracker dyes) mitochondrial membrane potential (e.g., tetramethylrho-damine methyl ester, TMRM tetramethylrhodamine ethyl ester, TMRE)... 

Cytotoxic tests were performed using two cell lines and the MTT [3, (4,5-dimethylthiazoyl-2-yl) 2,5 (diphenyl-tetrazolium bromide] rapid colorimetric assay. Cytotoxicity test with MTT showed no enhancement of cytotoxicity by modified proteins and their peptic and tryptic peptides against COS-7 and HL-60 cells after glycation. 

A tetrazolium salt (MTT) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide is able to enter cells and therefore assay levels of superoxide, without disrupting the cell with DMSO, forming the insoluble formazan product (Slater et al., 1963). Like the NBT test, MTT reduction is not specific for superoxide. 

Assessment of cytotoxicity MTT [3-(4,5-s-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (Dojindo, Kumamoto, Japan), Sodium dodecyl sulfate (SDS) (Wako Pure Chemical Industries), Microplate reader (POWERSCAN HT Dainippon Pharmaceutical, Osaka, Japan). 

The EpiOcular model uses normal human-derived epidermal keratinocytes, which form a stratified squamous epithelium morphologically similar to that found in the cornea [36]. The main endpoint evaluated is cell viability measured by dehydrogenase conversion of the vital dye MTT (3-(4,5-Dimethyl-2-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, Thiazolyl blue EINECS number 206-069-5, CAS number 298-93-1), into a blue formazan salt that is quantitatively measured after extraction from tissues. Two different protocols exist, the EpiOcular ET50 mainly developed for the testing of surfactants and surfactant-based mixtures, and the EpiOcular Eye Irritation Test (EIT) developed for a variety of chemistries. 

MSCB (master seed cell bank) 746 MTHFR, see methylenetetrahydrofolate reductase MTT (3,4,5-dmethylthiazol-2yl-2,5-diphenyl tetrazolium bromide) 640 Mucolytic enzymes 1376 Mucopolysaccharidosis 24, 862 Multiangle light scattering (MALS) 162 Multilamellae vesicles 1370 Multifunctional-type nanodevice (MEND) 1522... 

Colorimetric MTT (tetrazolium) assay (Mosmann 1983) was used to quantify living cells. In short, yellow MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) is reduced to purple colored formazan in the mitochondria of living cells. The absorbance of the colored solution is analysed using a spectrophotometer allowing for quantification of living cells. 

EFFECT OF STE ON CELL VIABILITY AS DETERMINED BY MTT, 3-[4,5-DIMETHYLTHIAZOL-2-YL]-2,5-DIPHENYL TETRAZOLIUM BROMIDE, ASSAY... 

Cell viability was evaluated using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) (Sigma Chemical Co.) assay. After culture, cells were treated with 1 mg/ml MTT for 2 h, precipitated dye was solubilized in dimethylsulfoxide, and the absorbance at 570 run was measured. 

WST-l Intracellular enzymes activity 2,5-diphenyl tetrazolium bromide) (colorimetric) XTT- MTS Alamar Blue Reduction of resazurin in metabolically active cells Fluorescence... 

Corrosive materials are identified by their ability to produce a deaease in cell viability below defined threshold levels at specified exposure periods, as determined using the MTT dye [3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyl tetrazolium bromid] reduction assay. Skin models such as EPISKIN (NIH, 2002) and EpiDerm (ECVAM, 2001) are used. 

Thiazolyl blue tetrazolium bromide (MTT, 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2//-tetrazolium bromide) [298-93-1, 2348-71-2] M 414.3, m 171°. It is recrystd by dissolving in MeOH containing a few drops of HBr and then adding dry Et20 to complete the crystn, wash the needles with Et20 and dry in a vacuum desiccator over KOH. [Beyer and Pyl Chem Ber 87 1505 7954.]... 

Cell proliferation inhibition. Cytotoxicity was determined as described previously34 by the colorimetric MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (Fluka) microculture assay on compounds freshly dissolved in DMSO... 

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