Thiazolyl Blue

Thiazolyl blue tetrazolium bromide (MTT, 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2//-tetrazolium bromide) [298-93-1, 2348-71-2] M 414.3, m 171°. It is recrystd by dissolving in MeOH containing a few drops of HBr and then adding dry Et20 to complete the crystn, wash the needles with Et20 and dry in a vacuum desiccator over KOH. [Beyer and Pyl Chem Ber 87 1505 7954.]... 

MTT solution 1 mg thiazolyl blue tetrazolium bromide (MTT Sigma-Aldrich) per ml and 5 mg/ml glucose in Dulbecco s PBS solution (solution must be stored at -20°C). 

The EpiOcular model uses normal human-derived epidermal keratinocytes, which form a stratified squamous epithelium morphologically similar to that found in the cornea [36]. The main endpoint evaluated is cell viability measured by dehydrogenase conversion of the vital dye MTT (3-(4,5-Dimethyl-2-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, Thiazolyl blue EINECS number 206-069-5, CAS number 298-93-1), into a blue formazan salt that is quantitatively measured after extraction from tissues. Two different protocols exist, the EpiOcular ET50 mainly developed for the testing of surfactants and surfactant-based mixtures, and the EpiOcular Eye Irritation Test (EIT) developed for a variety of chemistries. 

In this reaction sequence thiazolyl blue is measured since Ag-ae-NAD+ concentrations are small compared to the for NAD. Malic or alcohol dehydrogenase may also be used with Ag-aeNAD+, with a considerably higher cycling speed than the combination above. The detectability may be improved with bacterial luciferase (Schroe-der et al., 1976) ... 

Dimethoxyhydroxyphenylflurone has been proposed as a chromogenic reagent for the spectrophotometric determination (e = 1.3610 1 mof cm ) of trace amounts of Mo in steel and pure iron [4]. Thiazolylazo compounds were used as chromogenic reagents to determine Mo in human urine [5]. Thiazolyl blue tetrazolium bromide and 3,4,5-trihydroxybenzoic acid and nitrobluetetrazolium chloride were employed to determine Mo in soil [6,7]. 

Enzymatic Synthesis.— The phosphorimidazolidate method may be used tn prepare [y- PJATP. However, an enzymatic method which permits synthesis of [y- P]ATP with specific activity as high as 1 Ci //mol seems likely to be preferred (Scheme 1). The system involved has been used for a nximber of years but has been improved by adding oxidizing dyes - thiazolyl blue or phenazine methosulphate - to oxidize the NADH produced in the first step of... 

Methylthiazoletetrazolium NSC 60102 Methylthiazolyl-diphenyl Tetrazolium Thiazolyl Blue Monotetrazolium Thiazolyl blue Thiazolyl blue (Sigma) Thiazolyl blue tetrazolium bromide... 

The inhibitory activities of test compounds were measured by the enzymatic reaction. PDHc-El activity was measured by monitoring the absorbance at 566 nm wave length using a microplate reader (BioTek Synergy2, USA). The total final volume of 100 pL reaction mixture contained 50 mM K3PO4 (pH 7.0), 1.0 mM MgCla, 0.2 mM thiamine pyrophosphate (TPP), 0.5 mM thiazolyl blue (MTT),... 

Strictly, the name Thiazolyl blue applies to the bromide. Bromide ... 

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