Thiazolyl blue tetrazolium

Thiazolyl blue tetrazolium bromide (MTT, 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2//-tetrazolium bromide) [298-93-1, 2348-71-2] M 414.3, m 171°. It is recrystd by dissolving in MeOH containing a few drops of HBr and then adding dry Et20 to complete the crystn, wash the needles with Et20 and dry in a vacuum desiccator over KOH. [Beyer and Pyl Chem Ber 87 1505 7954.]... 

MTT solution 1 mg thiazolyl blue tetrazolium bromide (MTT Sigma-Aldrich) per ml and 5 mg/ml glucose in Dulbecco s PBS solution (solution must be stored at -20°C). 

Dimethoxyhydroxyphenylflurone has been proposed as a chromogenic reagent for the spectrophotometric determination (e = 1.3610 1 mof cm ) of trace amounts of Mo in steel and pure iron [4]. Thiazolylazo compounds were used as chromogenic reagents to determine Mo in human urine [5]. Thiazolyl blue tetrazolium bromide and 3,4,5-trihydroxybenzoic acid and nitrobluetetrazolium chloride were employed to determine Mo in soil [6,7]. 

Methylthiazoletetrazolium NSC 60102 Methylthiazolyl-diphenyl Tetrazolium Thiazolyl Blue Monotetrazolium Thiazolyl blue Thiazolyl blue (Sigma) Thiazolyl blue tetrazolium bromide... 

The EpiOcular model uses normal human-derived epidermal keratinocytes, which form a stratified squamous epithelium morphologically similar to that found in the cornea [36]. The main endpoint evaluated is cell viability measured by dehydrogenase conversion of the vital dye MTT (3-(4,5-Dimethyl-2-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, Thiazolyl blue EINECS number 206-069-5, CAS number 298-93-1), into a blue formazan salt that is quantitatively measured after extraction from tissues. Two different protocols exist, the EpiOcular ET50 mainly developed for the testing of surfactants and surfactant-based mixtures, and the EpiOcular Eye Irritation Test (EIT) developed for a variety of chemistries. 

A colorimetric version of the method uses a tetrazolium salt. The oxidation of the NADH is coupled to the reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide by a diaphorase (EC 1.6.4.3) and a deep blue formazan develops. The absorbance is read at a wavelength between 540 and 600 nm. This is more sensitive than the ultraviolet method but is more prone to interference, especially from any reducing agents present in the sample, which will result in a positive error. 

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