Differential Interference Contrast DIC microscopy

This provides a better contrast than the phase-contrast method by utilizing a phase difference to improve contrast. However, separation and recombination of the light beam into two beams is accomplished using prisms. DIC microscopy generates interference colours, and the contrast effects indicate the refractive index difference between the particle and medium. 

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A EXPERIMENTAL FIGURE 5-44 Live cells can be visualized by microscopy techniques that generate contrast by interference. These micrographs show live, cultured macrophage cells viewed by bright-field microscopy (/eft), phase-contrast microscopy middle), and differential interference contrast (DIC) microscopy righti. In a phase-contrast image, cells... 

To simultaneously examine epithelial closure and hemocyte recruitment Differential Interference Contrast (DIC) microscopy can be used, which will give a general wound outline. 

In video microscopy, for instance, background is normally subtracted using differential interference contrast (DIC) [18]. This technique, which requires a number of manipulations from the user, may now be automated using a new method called polarization-modulated (PMDIC) [19,20], It requires the introduction of a liquid crystal electro-optic modulator and of a software module to handle difference images. PMDIC has been shown to bring improvements in imaging moving cells, which show a low contrast, as well as thick tissue samples. 

Contrast. See also Differential interference contrast (DIC) computer-assisted, 16 487 in microscopy, 16 474 techniques for improving, 16 474-487... 

Nomarski microscopy is an examination mode using differential interference contrast (DIC). The images that DIC produces are deceptively three-dimensional with apparent shadows and a relief-like appearance. Nomarski microscopy also uses polarized light with the polarizer and the analyzer arranged as in the polarized light mode. In addition, double quartz prisms ( Wollaston prisms or DIC prisms) are used to split polarized light and generate a phase difference. 

Then, microscopic examinations follow optical research microscopes allow to determine the number, thickness, and color sequence of layers in paint fragments, and to recognize the textures as well as fundamental features of pigment and extender mixtures. Bright field and dark field illuminations, polarized light microscopy (incident and transmitted), particularly the differential interference contrast (DIC) procedure, and fluorescence microscopy are necessary for paint examinations (see Figure 3(A)-3(E)). 

Nomarski Differential Interference Contrast (DIC). In 1955 the physicist George Nomarski simplified the two-beam interference microscope in a way that it became available for routine microscopy [11 (Fig, 6), DIC uses modified Wollaston prisms (Fig. 6B). lying outside the focal... 

However, the most widely used type of interference microscopy is that giving Differential Interference Contrast (DIC), widely known as Nomarski contrast. 

FIGURE 10.1 Sacchammyces cerevisiae cells differential interference contrast (DIG) microscopy photograph of the yeast S. cerevisiae showing budded and unbudded cells (http // commons. wikimedia.oig/wiki/File S cerevisiae under DIC miciDscopyjpg). 

DCT DIC DICOM DIM DWT Discrete Cosine Transformation Differential Interference Contrast Digital Imaging Communications in Medicine Diffraction Imaging Microscopy Discrete Wavelet Transform... 

Hie most important optical technique for examining semiconductor wafer surfaces is the differential interference contrast microscopy method of Nomarski (N-DIC). First described in 1952, DIG... 

R. D. Allen et al. Video-Enhanced-Contrast. Differential Interference Contrast (AVEC-DIC) Microscopy A New Method Capable of Analyzing Microtubule-Related Motility in the Reticulopodial Network of Allogromia Laticollaris, Cell Motil. 1 (1981) 291-302. 

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