DIC microscopy

Figure 7. Video-enhanced DIC microscopy of rat liver Golgi apparatus membrane networks moving along microtubules using Xenopus egg microtubule motors (Allan and Vale, 1994). Top panel membrane extension with a bulbous terminus (arrow) attached to a microtubule (arrow heads). Middle panel same field two seconds later. The membrane has advanced about 3 pm along the microtubule (arrow). Bottom panel membrane has now advanced further along the microtubule (arrow). Bar = 2 pm.

This provides a better contrast than the phase-contrast method by utilizing a phase difference to improve contrast. However, separation and recombination of the light beam into two beams is accomplished using prisms. DIC microscopy generates interference colours, and the contrast effects indicate the refractive index difference between the particle and medium. 

A EXPERIMENTAL FIGURE 5-44 Live cells can be visualized by microscopy techniques that generate contrast by interference. These micrographs show live, cultured macrophage cells viewed by bright-field microscopy (/eft), phase-contrast microscopy middle), and differential interference contrast (DIC) microscopy righti. In a phase-contrast image, cells... 

The cells are examined by DIC and fluorescence microscopy. Cells (1-2 pL) are spotted onto a microscope slide and a cov-erslip is placed on the drop. The slides are viewed by DIC microscopy (to locate cells) and fluorescence microscopy with... 

Key words DTC, Cell migration, C. elegans, RNAi, DIC microscopy, Fluorescence microscopy... 

Prepare a wet mount slide for DIC microscopy. Melt 2% agarose in H O by microwaving, and place a 1-cm diameter drop in the center of a microscope slide. 

To simultaneously examine epithelial closure and hemocyte recruitment Differential Interference Contrast (DIC) microscopy can be used, which will give a general wound outline. 

R. D. Allen et al. Video-Enhanced-Contrast. Differential Interference Contrast (AVEC-DIC) Microscopy A New Method Capable of Analyzing Microtubule-Related Motility in the Reticulopodial Network of Allogromia Laticollaris, Cell Motil. 1 (1981) 291-302. 

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