Detergents cholate

The radioactively labeled proteins of the mitochondrial membranes were separated using an amphipathic chromatographic system The stationary phase of this system is polymethacrylic acid resin, some of the carboxylic groups of which are linked to oleylamine by means of an amide bond (as outlined in Fig. 3). The mobile phase is a solution of the detergents cholate and deoxycholate. Thus, the mobile phase and the resin... 

In experiments in which RCI and RCII of the brown seaweed Acrocarpia paniculata were exposed to the steroid detergents cholate or deoxycholate (<0.1% w/v) no shifts of the A were seen. In contrast, when either RCI or RCII were exposed to the same concentration of detergents having long alkyl tails, Triton X-100,LDAO,Zwittergens or Tweens then shifts to the blue of certain A occured (Barrett, unpublished). The A of... 

The aggregation behavior of C21-DA salt in dilute electrolyte medium appears to resemble that of certain polyhydroxy bile salts (25,16). That C21-DA, with a structure quite different from bile acids, should possess solution properties similar to, e.g., cholic acid is not entirely surprising in light of recent conductivity and surface tension measurements on purified (i.e., essentially monocarboxylate free) disodium salt aqueous solutions, and of film balance studies on acidic substrates (IX) The data in Figure 3 suggest that C21-DA salt micelles Incorporate detergents - up to an approximate weight fraction of 0.5 -much like cholate Incorporates lecithin or soluble... 

Detergents. Under appropriate conditions of pH, ionic strength and temperature, detergents (ionic sodium lauiyl sulphate, sodium deoxycholate, sodium cholate and cetyldiethyl-ammonium bromide, or nonionic Tweens and Tritons), can be used to lyse cells. Detergents may however cause enzyme inactivation and may need to be removed before purification. 

A modified biuret reagent was formulated (sodium tartrate replaces sodium potassium tartrate, the sodium hydroxide concentration is reduced, and potassium iodide was deleted). When the modified biuret reagent was mixed with samples containing 2% detergent (SDS or sodium cholate or Triton X-I00), it resulted in less protein-to-protein variation among six proteins. 

Detergents (26, 41, 46, 96, 97) Optimal concentrations of deoxycholate, cholate, Triton X-100, and cetyltri-methylammonium bromide activate, as does urea. Activation of phosphotransferase > that of phosphohydrolase. Supraoptimal levels inhibit, as do all tested concentrations of sodium lauryl sulfate and Tweens 20 and 80. (See also Lysolecithin, Fatty acids, and Long-chain fatty acyl-CoA esters, above)... 

Treatment of total human plasma proteins with sodium cholate and subsequent removal resulting in remodeling of the lipoproteins. See Pownall, H.J., Remodeling of human plasma lipoproteins by detergent perturbation. Biochemistry 44, 9714-9722, 2005. 

In the presence of an EOF, however, they migrate slowly towards the cathode. If now compounds are introduced into the capillary that interact with the SDS micelles, the mobility of these compounds will be influenced by the movement of the micelles to an extent that depends on the strength of this interaction. MECC is carried out using various forms of detergent anionic (e.g., SDS or Na-cholate), cationic (e.g., cetyltrimethylammonium bromide, CETB) and neutral (e.g., Triton X-100). 

Many different methods [340] are available for the reconstitution of bacteriorhodopsin into liposomes (a) sonication of purple membrane with dried, dispersed lipids [341] (b) co-precipitation of lipids and bacteriorhodopsin from organic solvents, such as dimethylsulfoxide [340] (c) dispersion of purple membrane sheets or monomeric bacteriorhodopsin and lipids in detergents, such as Triton X-100 [340], cholate [17,325], deoxycholate [170], or octyl-glucoside [327,342], followed by dialysis or removal of the detergent with Bio-Beads. 

Other conversion methods depend on the disruption of micelles containing water-insoluble compounds. For example, lipids e.g. lecithins can be reasonably well cosolubilized in micelles of sodium cholate or SDS. Dialysis slowly removes monomeric detergent molecules which dissociate away from the micelle resulting in a more concentrated lipid. Time and temperature controlled dialysis of the micelles finally yields monolamellar vesicles of uniform radii. ... 

The detergent sodium cholate (see Note 6) is added at a ratio of total lipids to detergent of 0.6 mol, including the lipophilic drug. 

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