Detectors chemiluminescent nitrogen detector

Supercritical fluid chromatography (SEC) was first reported in 1962, and applications of the technique rapidly increased following the introduction of commercially available instrumentation in the early 1980s due to the ability to determine thermally labile compounds using detection systems more commonly employed with GC. However, few applications of SEC have been published with regard to the determination of triazines. Recently, a chemiluminescence nitrogen detector was used with packed-column SEC and a methanol-modified CO2 mobile phase for the determination of atrazine, simazine, and propazine. Pressure and mobile phase gradients were used to demonstrate the efficacy of fhe fechnique. 

CLND Chemiluminescence nitrogen detector Pyro-chemiluminescence... 

Bhattachar, S.N., Wesley, J.A., and Seadeek, C. 2006. Evaluation of the chemiluminescent nitrogen detector for solubility determinations to support drug discovery. J. Pharm. Biomed. Anal. 41 152. 

Yan, B. Zhao, J. Leopold, K. Zhang, B. Jiang, G. Structure-Dependent Response of a Chemiluminescence Nitrogen Detector for Organic Compounds with Adjacent Nitrogen Atoms Connected by a Single Bond. Anal. Chem. 2007, 79, 718-726. 

EM Fujinari, JD Manes. Nitrogen-specific detection of peptides in liquid chromatography with chemiluminescent nitrogen detector. J Chromatogr A 676 113-120, 1994. 

FIGURE 10.2 Mechanism for the chemiluminescent nitrogen detector. R = any group attached to a nitrogen. 

Fujinary E, Manes JD, Bizanek R, Peptide content determination of crude synthetic peptides by reversed-phase liquid chromatography and nitrogen-specific detection with a chemiluminescent nitrogen detector, J. Chromatogr A, 743 85-89, 1996. 

Nitrogen a Dohrmann microcoulometric reactor being used in reductive mode was replaced with an Antek Model 771 pyroreactor which uses a chemiluminescent nitrogen detector. 

E. W. Taylor, W. Jia, M. Bush, and G. D. Dollinger, Accelerating the drug optimization process Identification, structure elucidation, and quantification of in vivo metabolites using stable isotopes with LC/MS" and the chemiluminescent nitrogen detector, Anal Chem. 74 (2002), 3232-3238. 

Taylor, E.W. Jia, W. Bush, M. Dollinger, G.D. Accelerating the Drug Optimization Process Identification, Structure Elucidation, and Quantification of In Vivo Metabolites using stable isotopes with LC/MSn and the chemiluminescent nitrogen detector, AnaZ. Chem. 74(13), 3232-3238 (2002). 

Deng, Y. et al., Quantitation of drug metabolites in the absence of pure metabolite standards by high-performance liquid chromatography coupled with a chemiluminescent nitrogen detector and mass spectrometer, Rapid Commun. Mass Spectrom., 18, 1681, 2004. 

Recently, new analytical techniques incorporating instruments such as the evaporative light-scattering detector (ELSD) [36] and the chemiluminescent nitrogen detector (CLND) [37] have begun to be used to address the issue of quantitative analysis. Due to the relative novelty of these methods in their application to combinatorial chemistry, we will spend some time describing them in more detail. 

SPTD short-path thermal desorption unit CLND chemiluminescent nitrogen detector CL peroxyoxalate chemiluminescence detection CELL continuous liquid-liquid extraction. 

Thi,s chemiluminescent nitrogen detector for HPLC was first described in (23). The detection mechanism for nitrogen determination is shown below ... 

Determination of ppm concentrations of nitriles in light naphtha was carried out by capillary GC, by either of the following procedures. Method 1 Clean-up and preconcentration by medium pressure LC removed the hydrocarbons and increased the nitrile concentration ten fold the samples were analysed using an FI detector. Method 2 Direct injection into a capillary GC using a chemiluminescence nitrogen detector ... 

Looking for potential undetected peaks (non-chromophoric degradant) by alternative separations and/or detection such as MS [39], infrared spectroscopy [49], refractive index, chemiluminescence nitrogen detector, TLC (with U or acid/charring visualization), CAD, or ELSD... 

Here we will describe several approaches of quantitative analysis of combinational libraries in Affymax, including QC of libraries by chemiluminescent nitrogen detector (CLND), quantitative analysis by standard bead LCVMS method, and estimation of concentration from UV response. 

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