Nitrogen specific detection

The sample is homogenized with acetonitrile. An aliquot of the extract is evaporated to dryness and the residual material is dissolved in ethyl acetate-toluene (3 1, v/v), and subjected to cleanup by gel permeation chromatography (GPC). After GPC, the sample is subjected to an alumina and Florisil SPE cleanup procedure. The concentrated eluate is analysed by gas chromatography/thermionic nitrogen-specific detection (GC/TSD). 

Nitrophenols in fog and atmospheric particles were determined by GC of the underiva-tized compounds and their corresponding acetate esters. Four fused-silica columns were used with three alternative detection modes, namely mass-selective detection, nitrogen-specific detection and ECD. GC-ECD of the acetate derivatives gave the best results501. A capillary GC-UVD method was developed for the determination of small amounts of nitrophenols present in the environment. The method was compared with HPLC-UVD from the point of view of selectivity and sensitivity. LOD for GC were about one-tenth of those for HPLC502. 

Nitromethane can be determined in workplace air by adsorbing the air sample on Chromosorb, desorbing with ethyl acetate and analysing by gas chromatography with nitrogen-specific detection (method 2527) (Eller, 1994). 

Lawrence JF, Iverson F. 1975. Analysis of the diazinon metabolites G 27550 and GS 31144 by gas liquid chromatography with nitrogen-specific detection after derivatization. J Chromatogr 103 341-347. 

EM Fujinari, JD Manes. Nitrogen-specific detection of peptides in liquid chromatography with chemiluminescent nitrogen detector. J Chromatogr A 676 113-120, 1994. 

RR Laski, RR Watts. Gas chromatography of organonitrogen pesticides, using a nitrogen-specific detection system. J Assoc Off Anal Chem 56 328-332, 1973. 

Hormann et al. [157] monitored various European rivers for levels of Atrazine, Terbumeton and dealkylated metabolites GS26571 (2-amino-4-tertbutylammo-5-methoxy-l,3,5-triazine) and GS30033 (2-amino-4-chloro-5-ethylamino-l,3,5-triazine). The compounds were extracted into dichloromethane and quantitated by gas chromatography with nitrogen-specific detection. Selected results were verified by gas chromatography with mass fragmentographic detection. The limit of detection was usually 0.4pg L 1. 

Fujinary E, Manes JD, Bizanek R, Peptide content determination of crude synthetic peptides by reversed-phase liquid chromatography and nitrogen-specific detection with a chemiluminescent nitrogen detector, J. Chromatogr A, 743 85-89, 1996. 

This assay is used for the quantitative determination of the angeotensin converting enzyme (ACE) inhibitor ramipril, (2-[N-[(S)-l-Ethoxycarbon-yl-3-phenylpropyl]-L-alanyl]-(lS,3S,5S)-2-azabicy-clo[3.3.0]octane-3-carboxylic acid), and its pharmacologically active metabolite, ramiprilat, in human urine using capillary gas chromatography with nitrogen specific detection (NPD). 

Ling, C. F., Perez-Melian, G., Jimenez-Conde, F., and Revilla, E., Comparative study of some columns for direct determination of carbofuran by gas chromatography with nitrogen-specific detection, J. Chromatogr., 519, 359-362, 1990. 

Determination of Unchanged Hydralazine in Plasma by Gas-Liquid Chromatography Using Nitrogen-Specific Detection... 

Sensitive GLC Assay for Pemoline in Biological Fluids Using Nitrogen-Specific Detection J. Pharm. Sci. 68(4) 445-447 (1979) ... 

Liskevych, U., and David, J. M. Determination of Butaperazine in Biological Fluids by Gas Chromatography Using Nitrogen Specific Detection System... 

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