Chemiluminescent nitrogen detector

Supercritical fluid chromatography (SEC) was first reported in 1962, and applications of the technique rapidly increased following the introduction of commercially available instrumentation in the early 1980s due to the ability to determine thermally labile compounds using detection systems more commonly employed with GC. However, few applications of SEC have been published with regard to the determination of triazines. Recently, a chemiluminescence nitrogen detector was used with packed-column SEC and a methanol-modified CO2 mobile phase for the determination of atrazine, simazine, and propazine. Pressure and mobile phase gradients were used to demonstrate the efficacy of fhe fechnique. 

CLND Chemiluminescence nitrogen detector Pyro-chemiluminescence... 

Gas chromatography is one of the most powerful analytical techniques available for chemical analysis. Commercially available chemiluminescence detectors for GC include the FPD, the SCD, the thermal energy analysis (TEA) detector, and nitrogen-selective detectors. Highly sensitive detectors based on chemiluminescent reactions with F2 and active nitrogen also have been developed. 

Bhattachar, S.N., Wesley, J.A., and Seadeek, C. 2006. Evaluation of the chemiluminescent nitrogen detector for solubility determinations to support drug discovery. J. Pharm. Biomed. Anal. 41 152. 

Yan, B. Zhao, J. Leopold, K. Zhang, B. Jiang, G. Structure-Dependent Response of a Chemiluminescence Nitrogen Detector for Organic Compounds with Adjacent Nitrogen Atoms Connected by a Single Bond. Anal. Chem. 2007, 79, 718-726. 

EM Fujinari, JD Manes. Nitrogen-specific detection of peptides in liquid chromatography with chemiluminescent nitrogen detector. J Chromatogr A 676 113-120, 1994. 

Nussbaum MA, Baertschi SW, Jansen PJ. Determination of relative UV response factors for HPLC by use of a chemiluminescent nitrogen-specific detector. J Pharm Biomed Anal 2002 27 983-993. 

FIGURE 10.2 Mechanism for the chemiluminescent nitrogen detector. R = any group attached to a nitrogen. 

Fujinary E, Manes JD, Bizanek R, Peptide content determination of crude synthetic peptides by reversed-phase liquid chromatography and nitrogen-specific detection with a chemiluminescent nitrogen detector, J. Chromatogr A, 743 85-89, 1996. 

Nitrogen a Dohrmann microcoulometric reactor being used in reductive mode was replaced with an Antek Model 771 pyroreactor which uses a chemiluminescent nitrogen detector. 

Many other on-line detectors suitable for SEC columns as reviewed [154], including chemiluminescent nitrogen detection, dynamic surface tension detection, high frequency detection and Fourier transform infrared detection, can be applied to FFF the latter being capable of delivering polymer compositions online. 

E. W. Taylor, W. Jia, M. Bush, and G. D. Dollinger, Accelerating the drug optimization process Identification, structure elucidation, and quantification of in vivo metabolites using stable isotopes with LC/MS" and the chemiluminescent nitrogen detector, Anal Chem. 74 (2002), 3232-3238. 

Additional detection techniques that can be employed to help solve mass balance issues with RP-HPLC are MS [30], chemiluminescent nitrogen-specihc detector [31], evaporative light-scattering detector, ELSD [32], and corona charged aerosol detection [CAD] [33],... 

Two other types of element-specific detector for nitrogen currently in use coupled to SFCs are the nitrogen phosphorus detector (NPD) and the thermal energy analyzer (TEA). The NPD uses a hot, catalytically active solid surface immersed in a layer of dissociated H2 and O2 to form electronegative N and P ions which are detected on a nearby electrode [2]. NPD has been shown to have broad application in SFC, especially in the agrochemical industry [3]. The TEA, as described by Fine et al. [4], uses low-temperature pyrolysis, followed by ozone-induced chemiluminescence, for the detection of compounds containing NO2 groups. The TEA has been used for the determination of tobacco-specific nitrosamines and explosives [5]. Both of these detectors require spedlic standards of the analytes of interest for quantitation... 

Taylor, E.W. Jia, W. Bush, M. Dollinger, G.D. Accelerating the Drug Optimization Process Identification, Structure Elucidation, and Quantification of In Vivo Metabolites using stable isotopes with LC/MSn and the chemiluminescent nitrogen detector, AnaZ. Chem. 74(13), 3232-3238 (2002). 

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