Detector high-performance liquid chromatographic

Gas chromatograph equipped with an electron capture detector High-performance liquid chromatograph... 

Coupling of analytical techniques (detectors) to high-performance liquid chromatographic (HPLC) systems has increased in the last tree decades. Initially, gas chromatography was coupled to mass spectrometry (MS), then to infrai ed (IR) spectroscopy. Following the main interest was to hyphenate analytical techniques to HPLC. 

High-performance liquid chromatograph equipped with a UV detector Microsyringe, 25- iL... 

High-performance liquid chromatograph Model LC-6A equipped with RF-535 fluorescence detector (Shimadzu Co., Japan)... 

Torsi, G., Chiavari, G., Laghi, C., and Asmudsdottir, A., Responses of different UV-visible detectors in high-performance liquid chromatographic measurements when the absolute number of moles of an analyte is measured, /. Chromatogr., 518, 135, 1990. 

High Performance Liquid Chromatographic (HPLC) Analysis. A Waters HPLC system (two Waters 501 pumps, automated gradient controller, 712 WISP, and 745 Data module) with a Shimadzu RF-535 fluorescence detector or a Waters 484 UV detector, and a 0.5 pm filter and a Rainin 30 x 4.6 mm Spheri-5 RP-18 guard column followed by a Waters 30 x 3.9 cm (10 pm particle size) p-Bondapak C18 column was used. The mobile phase consisted of a 45% aqueous solution (composed of 0.25% triethylamine, 0.9% phosphoric acid, and 0.01% sodium octyl sulfate) and 55% methanol for prazosin analysis or 40% aqueous solution and 60% methanol for naltrexone. The flow rate was 1.0 mL/min. Prazosin was measured by a fluorescence detector at 384 nm after excitation at 340 nm (8) and in vitro release samples of naltrexone were analyzed by UV detection at 254 nm. 

Nagels, L.J., Creten, W.L. (1985). Evaluation of the glassy carbon electrochemical detector selectivity in high-performance liquid chromatographic analysis of plant material. Anal. Chem. 57, 2706. 

Fig. 18b.1. Electrochemical cells and representative cell configurations, (a) Schematic diagram of a cell-potentiostat system, (b) Typical laboratory cell with Hg-drop electrode and drop knocker, (c) Voltammetric cell as detector at the end of a high-performance liquid chromatographic column, (d) A two-electrode (graphite) chip cell for biosensor development, (e) Three-electrode chip cells on a ceramic substrate for bioanalytical work.

A spectrofluorimeter has been used as a detector in the high-performance liquid chromatographic separation of polyaromatic hydrocarbons in water samples [12-17], A great improvement in sensitivity and specificity can be obtained by the correct wavelengths. 

High-performance liquid chromatographic separation with electrochemical detection may provide the best sensitivity for phenol quantification in biological samples. The use of gas chromatography with a flame ionization detector may be a more versatile method, if other non-ionic pollutants must be quantified. The advantages and disadvantages of different methods available for the quantification of phenol and metabolites in biological and environmental samples have been discussed by Tesarova and Packova(1983). 

Several recent high-performance liquid chromatographic separation schemes are applicable since they also incorporate detectors not usually associated with conventional hydrocarbon group analyses (Matsushita et al., 1981 Miller et al., 1983 Rawdon, 1984 Lundanes and Greibokk, 1985 Schwartz and Brownlee, 1986 Hayes and Anderson, 1987). 

Nogata, Y. et al.. High-performance liquid chromatographic determination of naturally occurring flavonoids in Citrus with a photodiode-array detector, J. Chromatogr., 667, 59, 1994. 

Often, treatment of samples with fluorescence labeling agent reacts with primary and secondary amines to give a fluorescent compound. This is especially important for detecting amino acids in protein hydrolyzates. Fluorescence detectors may also be integrated into a high performance liquid chromatographic (HPLC) system. 

High-performance liquid chromatograph (HPLC) with fluorescence detector (excitation 290 nm, emission 330 nm) or UV detector (295 nm)... 

High-performance liquid chromatograph (HPLC e.g., Waters Chromatography) equipped with column heater, solvent pump, UV detector (set at 210 nm), integrator, autosampler, and (for manual injection) a 10-pl sample loop 15 x 0.46-cm YMC-ODS-AQ analytical column (AQ12S031546WT, Waters Chromatography)... 

FD Conforti, CH Harris, JT Rinehart. High performance liquid chromatographic analysis of wheat flour lipids using an evaporative light scattering detector. J Chromatogr 645 83-88, 1993. 

KM Weaver, ME Neale. High performance liquid chromatographic detection and quantitation of synthetic acid fast dyes with a diode array detector. J Chromatogr 3 486-489, 1985. 

Because of this concern, a variety of methods have been developed for the determination of NOC in foods. These include thin-layer chromatographic (TLC) (11,12), gas chromatographic (GC) (13-15), GC-mass spectrometric (GC-MS) (15-17), and high-performance liquid chromatographic (HPLC) (18-20) techniques. The purpose of this article is to review various HPLC methods developed for this purpose. Unfortunately, however, only limited advances have been made in this area, mainly because of the lack of sensitive and specific detectors. Most published methods for NOC reported to date are GC-based techniques. Therefore, this review will be a brief one and will emphasize the most recent HPLC developments. 

The end products of acid and enzymatic hydrolysis of corncob and sugarcane bagasse were analyzed by using a high-performance liquid chromatograph (Waters, Milford, MA) equipped with a Rheodyne automatic injector with a 20- iL injection capacity loop, a Shodex Sugar SC 1011 column, and an integrator model 747 with a model 410 RI detector. The mobile phase was deionized water, and the flow rate was adjusted to 0.8 mL/min. 

G. W. Schieffer, Limitation of assessing high-performance liquid chromatographic peak purity with photodiode array detectors, J. Chromatogr., 379 387 (1985). 

The basic components of a high-performance liquid chromatographic system are shown in Figure 3.1. The instrument consists of (a) eluent containers for the mobile phase, (b) a pump to move the eluent and sample through the system, (c) an injection device to allow sample introduction, (d) a column(s) to provide solute separation, (e) a detector to visualize the separated components, (f) a waste container for the used solvent, and finally (g) a data collection device to assist in interpretation and storage of results. 

A high performance liquid chromatographic procedure has been described [3] for the simultaneous determination of the fungicides sodium-A-methyldithiocarbamatc and methylisothiocyanate in surface waters and in sewage, based on their separation on a reversed phase column with a miscellar mobile phase (hexadecyltrimethylammonium bromide) in 1 1 v/v methanohwater buffered to pH6.8. Detection Limits were 70 and lpg dm 1 when the analysis was performed with an ultraviolet detector at 247nm. 

Williams et al. used a high performance liquid chromatographic assay method for dipyridamole monitoring in plasma [71]. The HPLC system uses a Waters model 6000 A solvent delivery pump equipped with a U6K injector, a pBondapak C 9 column (30 cm x 39 mm 10 pm), and a Model 440 absorbance detector. The signal from the detector was quantified using a Shimadzu data processor and an Omni-Scribe recorder. A mobile phase flow rate of 1.5 mL/min was produced by a pressure of approximately 102 atm (1500 p.s.i.). The mobile phase was 50 50 mixture of acetonitrile and 0.01 M sodium phosphate in water (adjusted to pH 7). The absorbance reading of dipyridamole in methanol was made at 280 nm. 

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