Chromatographic analysis high-performance liquid

Chromatographic Methods of Analysis High Performance Liquid Chromatography... 

Chromatographic Methods of Analysis High Performance Liquid Chromatography, p. 526. Metabolite Identification in Drug Discovery,... 

Modern tHcy methods include enzyme immunoassays and chromatographic-based methods. In practice, immunoassays " are most often used for routine purposes (e.g., fluorescence polarization immunoassay IFPIA] as run on Abbott s IMx and AxSYM platforms) Chromatographic assays include amino acid analysis high-performance liquid chromatography (HPLC) with ultraviolet, fluorescence, or electrochemical detection ° " capillary electrophoresis with fluorescence detection gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with tandem MS (MS-MS). 

Shibukawa, A. Terakita, A. He, J.Y. Nakagawa, T. High-performance frontal analysis-high-performance liquid chromatographic stem for stereoselective determination of unbound ketoprofen enantiomers in plasma after direct sample injection. J.Pharm.Sci., 1992,81, 710—715 [plasma column-switching column temp 15 chiral LOD 1 nM human rat]... 

Castello, G. and Gerbino, T. C., Analysis of polycyclic aromatic hydrocarbons with ion-trap mass detector and comparison with other gas-chromatographic and high-performance liquid-chromatographic techniques, J. Chromatogr. A, 642, 351-357, 1993. 

J. A. Apffel, T. V. Alfredson and R. E. Majors, Automated on-line multi-dimensional high performance liquid chromatographic techniques for the clean-up and analysis of water-soluble samples , J. Chromatogr. 206 43-57 (1981). 

Despite the difficulties caused by the rapidly expanding literature, the use of chiral stationary phases (CSPs) as the method of choice for analysis or preparation of enantiomers is today well established and has become almost routine. It results from the development of chiral chromatographic methods that more than 1000 chiral stationary phases exemplified by several thousands of enantiomer separations have been described for high-performance liquid chromatography (HPLC). 

For off-bead analysis, coupling between chromatographic separation and mass spectrometric detection has proven especially powerful. The combination between high performance liquid chromatography (HPLC) and electrospray ionisation mass spectrometry has the advantage that purity of product mixtures can be coupled on-line with the product identification. 

Strack, D. and Reznik, H., High-performance liquid chromatographic analysis of betaxanthins in Centrospermae (Caryophyllales), Ztschr. Pflanzenphysiol, 94, 163, 1979. 

Goodall, I., Dermis, J. J., Parker, 1., and Sharman, M. (1995). Contribution of high performance liquid chromatographic analysis of carbohydrates to authenticity testing of honey. 

Bogusz, M., Franke, J. P., de Zeeuw, R. A., and Erkens, M., An overview of the standardization of chromatographic methods for screening analysis in toxicology by means of retention indices and secondary standards. Part II. High performance liquid chromatography, Fresenius ]. Anal. Chem., 347, 73, 1993. 

Sharman, M. and Gilbert, J., Automated aflatoxin analysis of foods and animal feeds using immunoaffinity column clean-up and high-performance liquid chromatographic determination, /. Chromatogr., 543, 220, 1991. 

Stone, K. L. and Williams, K. R., High-performance liquid chromatographic peptide mapping and amino acid analysis in the subnanomole range, /. Chromatogr., 359, 203, 1986. 

---