Detectability of protein

Detection of Protein Damage Arising the Radical-mediated Peroxidation of ... 

Sandwich ELISAs (Eigure 4) are the most common type of immunoassay used for the detection of proteins. A capture antibody is immobilized on the wells of a microplate. The solution containing the analyte is introduced and antibody-analyte... 

Bacterial two-hybrid system for the detection of protein-protein interactions... 

Madonna, A. I Basile, F. Imma, I. Meetani, A. M. Rees, J. C. Voorhees, K. J. On-probe sample pretreatment for the detection of proteins above 15 KDa from whole cell bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Comm. Mass Spectrom. 2000,14, 2220-2229. 

Coldham, N. G. Woodward, M. J. Characterization of the Salmonella typhimurium proteome by semi-automated two dimensional HPLC-mass spectrometry Detection of proteins implicated in multiple antibiotic resistance. J. Proteome Res. 2004, 3,595-603. 

Recently, novel polymethine carbonyl-dyes based on coumarin moiety and their boron difluoride complexes 9a-d and lOa-d [34—36] were evaluated as fluorescent dyes for the detection of native proteins using bovine serum albumin (BSA) as a model protein, and as probes for the nonspecific detection of proteins using a BSA/ sodium dodecyl sulfate (SDS) mixture [37]. Optical properties of these compounds in the absence and presence of BSA, as well as in SDS and BSA/SDS mixture, were measured in Tris-HCl buffer (pH 8.0) (Table 1). 

Kovalska VB, Volkova KD, Manaev AV et al (2010) 2-Quinolone and coumarin poly-methines for the detection of proteins using fluorescence. Dyes Pigm 84 159-164... 

Kato, N., Pontier, D. and Lam, E. (2002). Spectral profiling for the simultaneous observation of four distinct fluorescent proteins and detection of protein-protein interaction via fluorescence resonance energy transfer in tobacco leaf nuclei. Plant Physiol. 129, 931-42. 

Song X., Swanson B.I., Direct, ultrasensitive, and selective optical detection of protein toxins using multivalent interactions, Anal. Chem. 1999 71 2097-2107. 

When cells are suspended in a biological fluid or culture medium, both serum proteins and cells interact with the surface substrate. Serum protein adsorption behavior on SAMs has been examined with various analytical methods, including SPR [58-61], ellipsometry [13, 62, 63], and quartz QCM [64—66]. These methods allow in situ, highly sensitive detection of protein adsorption without any fluorescence or radioisotope labeling. SPR and QCM are compatible with SAMs that comprise alkanethiols. In our laboratory, we employed SPR to monitor protein adsorption on SAMs. 

Bayer, E.A., and Wilchek, M. (1992) Labeling and detection of proteins and glycoproteins. In Nonradioactive Labeling and Detection of Biomolecules (C. Kessler, ed.), pp. 98-99. Springer-Verlag, New York. 

Chaudhuri, A.R., de Waal, E.M., Pierce, A., Van Remmen, H., Ward, W.F., and Richardson, A. (2007) Detection of protein carbonyls in aging liver tissue A fluorescence-based proteomic approach. Mech. Ageing Dev. 127(11), 849-861. 

LaRochelle, W.J., and Froehner, S.C. (1986b) Immunochemical detection of proteins biotinylated on nitrocellulose replicas./. Immunol. Meth. 92, 65-71. 

T.L. Lasseter, W. Cai, and R.J. Hamers, Frequency-dependent electrical detection of protein binding events. Analyst (Cambridge, United Kingdom) 129, 3-8 (2004). 

H. Ge, UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions. Nucl. Acids Res. 28, e3 (2000). 

P. Hazarika, B. Ceyhan, and C.M. Niemeyer, Sensitive detection of proteins using difunctional DNA-gold nanoparticles. Small 1, 844-848 (2005). 

In earlier studies the in vitro transition metal-catalyzed oxidation of proteins and the interaction of proteins with free radicals have been studied. In 1983, Levine [1] showed that the oxidative inactivation of enzymes and the oxidative modification of proteins resulted in the formation of protein carbonyl derivatives. These derivatives easily react with dinitrophenyl-hydrazine (DNPH) to form protein hydrazones, which were used for the detection of protein carbonyl content. Using this method and spin-trapping with PBN, it has been demonstrated [2,3] that protein oxidation and inactivation of glutamine synthetase (a key enzyme in the regulation of amino acid metabolism and the brain L-glutamate and y-aminobutyric acid levels) were sharply enhanced during ischemia- and reperfusion-induced injury in gerbil brain. 

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