3-Deoxyglucose

Mutation. For industrial appHcations, mutations are induced by x-rays, uv irradiation or chemicals (iiitrosoguanidine, EMS, MMS, etc). Mutant selections based on amino acid or nucleotide base analogue resistance or treatment with Nystatin or 2-deoxyglucose to select auxotrophs or temperature-sensitive mutations are easily carried out. Examples of useful mutants are strains of Candida membranefaciens, which produce L-threonine Hansenu/a anomala, which produces tryptophan or strains of Candida lipolytica that produce citric acid. An auxotrophic mutant of S. cerevisiae that requires leucine for growth has been produced for use in wine fermentations (see also Wine). This yeast produces only minimal quantities of isoamyl alcohol, a fusel oil fraction derived from leucine by the Ehrlich reaction (10,11). A mutant strain of bakers yeast with cold-sensitive metaboHsm shows increased stabiUty and has been marketed in Japan for use in doughs stored in the refrigerator (12). 

Note. The names 2-deoxyribose (for 2-deoxy-D-erythro-pentose) and 2-deoxyglucose (for 2-deoxy-D-araimo-hexose) are often used. 

Noland-Gerbec EA, Pfohl RJ, Taylor DH, et al. 1986. 2-Deoxyglucose uptake in the developing rat brain upon pre- and postnatal exposure to trichloroethylene. Neurotoxicology 7 157-164. 

The first lecture given by Tomas Hokfelt, who pioneered anatomical studies based on amine fluorescence, was entitled, Neuroanatomy for the Neurochemist while the second lecture given by Louis Sokoloff, who invented the methodology of functional brain imaging originally based on metabolism of radionuclide labeled-2-deoxyglucose, was entitled Neurochemistry for the Neuroanatomist . The history and further information... 

Astic, L. and Saucier, D. (1981) Ontogenesis of the functional activity of rat olfactory bulb autoradiographic study with the 2-deoxyglucose method. Brain Res. 254, 243-256. 

Endo-2-acetamido-2-deoxy-)3-D-glucosidase has been used in structural studies of glycopeptides.30 The enzyme cleaves the linkage between the two 2-amino-2-deoxyglucose residues that constitute the chitobiose residue linked to L-asparagine in glycoproteins containing... 

Seymour and Klinman (entry 6 in Table 2 see Fig. 5 for a schematic mechanism) measured relative rate constants for the hydride-transfer reaction (H, D, T) from Cl of 2-deoxyglucose to the cofactor FAD. To explore the model for enzymically enhanced mnneling, according to which the enzyme conducts a fluctuational search for an efficient mnneling sub-state, five variants of glucose oxidase, anticipated to have differing capacities for the fluctuational search, were generated. 

Volkow ND, Gillespie H, Mullani N, Tancredi L, Grant C, Ivanovic M, Hollister L. (1991). Cerebellar metabolic activation by delta-9-tetrahydrocannabinol in human brain a study with positron emission tomography and 18F-2-fluoro-2-deoxyglucose. Psychiatry Res. 40(1) 69-78. 

Aldose (D-xylose, o-galactose, o-mannose, D-2-deoxyglucose, D-glucosamine hydrochloride) (11.1 mmol)... 

Deoxyglucose (2DG) is a nonmetabolizeable carbon source that blocks glycolysis in concentrations around 50 mM (103). 

Brown J. Effects of 2-deoxyglucose on carbohydrate metabolism review of the literature and studies in the rat. Metabolism 1962 11 1098-1112. 

Specificity The transporter has a similar specificity to hexokinase it transports several hexoses, glucose, mannose, 2-deoxyglucose. Similarly, the optical isomer, D-glucose, but not L-glucose, is transported (Chapter 3). 

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