Quantitative autoradiographic 2-deoxyglucose technique

Modifications of the technique described by Sokoloff et al. (1977) are in more widespread use at the present time than is the original quantitative approach itself. In all cases, the modifications have been introduced in an attempt to simplify aspects of the original deoxyglucose technique (such as to eliminate the need to sample arterial blood with the attendant necessity of surgical intervention), although almost invariably at the expense of the scientific rigor of the original quantitative technique (Sokoloff et al., 1977). 

There is evidence that not only are the inherent assumptions of many of the semiquantitative approaches not wholly valid (see above), but that the measure itself (e.g., the ratio of optical density in the region relative to the optical density of white matter) may be altered by factors unrelated to glucose utilization. For example, the relationship of tissue isotope concentration to optical density is not linear, and the relationship varies with the exposure time in the preparation of the autoradiograms. As a 

Alternative Approaches for Determining Rates of Glucose Utilization 

In the technique described by Crane et al. (1978, 1980), the accumulation of deoxyglucose-6-phosphate over a brief period (4 min) following the sequential injection of [ H]- and [ C]deoxyglucose is determined following rapid inactivation of hexokinase (by means of microwave irra- 

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