Constraints of the Autoradiographic Deoxyglucose Technique

The value of the lumped constant is a major determinant of the amount of tracer retained in the brain during the experimental period. 

Two other constraints (the constancy of plasma glucose and of the rate of glucose utilization during the period of measurement) impose minor restrictions on experimental design. The recent derivation of a modified operational equation permits the deoxyglucose technique to be applied quantitatively to conditions in which the moderate, dynamic alterations in plasma glucose levels occur during the measurement of 

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