Delphinium barbeyi

Delphinium barbeyi (Huth) Huth, D. occi-dentaie (S. Wats) S. Wats. 

Deltaline Delphinium barbeyi. D. elatum, D. nACh-R antagonist (110) (all... 

Delphinium barbeyi, D. datum, D. spp. (larkspur) (Ranunculaceae) [root]... 

Fig- 13.20 Electrospray ionization mass spectrum of a crude methanol extract of Delphinium barbeyi plant material. The methanol extract was diluted with the electrospray flow solvent (50% methanol in 1 % acetic acid) and a 20 p.L loop injection... 

By specifically and selectively reconstructing the total ion peak observed after a loop flow injection to display only the m/z values of interest, the area count for that ion can be extracted from the composite total ion peak. The measured area count can then be used to estimate the quantity of specific alkaloid by comparison to a calibration curve. Thus, component compounds in a mixture are separated by mass, as opposed to chromatography, for quantitation. To normalize the variability of the API response, an internal reference standard is added to the sample prior to loop injection. Alkaloids for which standards are not available are reported as equivalents of a closely related and available standard used to generate the calibration curve, for example, deltaline and methyllycaconitine have been used as calibration standards to represent the non-MSAL and MSAL types of alkaloids, respectively, in the plant material. Calibration curves for these two compounds were linear (r > 0.990) and there appears to be no selective suppression of lower-level alkaloids (figure 13.21). Multiple analyses of Delphinium barbeyi samples returned a level ofprecision that was less than 10 % (relative standard deviation) for all components [56]. 

Fig. 13.23 Plot of the first three canonical variables from the discriminant analysis of the tabulated ESI-MS data for toxic Delphinium species according to Ewan [59]. Croups A and B are Delphinium barbeyi, group C is D. occidentale, group D is D. glaucum, and group E is a hybrid (barb, x occi.).

This paper described the chemotaxonomy characteristics of two C2o-diterpenoid alkaloids and five C 19-diterpenoid alkaloids from Delphinium albocoeruleum Maxim of Chinese origin. The study indicated that the compound delelatine may be considered as a chemotaxonomic marker for the Delphinium genus as it occurs in Delphinium elatum. Delphinium barbeyi, D. tatsinenense [66], and Delphinium giraldii [67], Delphinine, a major compound, may possibly be utihzed for identification of D. albocoeruleum Maxim. 

Pfister, J. A., Manners, G. D., Gardner, D. R., and Ralphs, M. H. 1994a. Toxic Alkaloid Levels in Tall Larkspur Delphinium barbeyi) in Western Colorado. Journal of Range Management 47 355-358. 

Inula royleana Delphinium consolida D. barbeyi Correlated with lycoctonine Refs. 3, 4, 132, 172, 173... 

Delphinium occidentale D. elatum D. barbeyi and others Chemical data Refs. 2, 3, 180... 

Some Delphinium species are poisonous to cattle and cause losses in livestock on mountain grazing ranges. Manners and Ralphs (1989) report the analysis of the alkaloid content of D. occidentale and D. barbeyi on a medium polarity column. Dictyocarpine and dictypocarpinine could not be separated under the conditions used and quantitative analysis of five alkaloids was shown to be possible. Figure 6 illustrates a GC separation of these compounds. 

Fig. 6. a Capillary GC trace of total alkaloid extract of Delphinium occidentale. b Capillary GC trace of total alkaloid extract of D. barbeyi. Peaks 1 Delpheline 2 deltamine 3 deltaline 4 14-acetyldictyocarpine 5,6 dictyocarpine and dictyocarpinine 7 delcosine. The GC conditions are described in Table 5. (Manners and Ralphs 1989)... 

---