Delphinium occidentale

Norditerpene Loline Pyrrolizidine Delphinium occidentale Lolium temulentum Castilleja sulphurea Rhinanthus minor... 

Many of the earliest applications of API-MS used direct syringe infusion of crude alkaloidal extracts into the ESI source. In this manner, Marko and Stermitz [55] were able to detect the transfer of alkaloids from Delphinium occidentale to the root parasitic Castilleja sulphurea. 

Delphinium occidentale D. elatum D. barbeyi and others Chemical data Refs. 2, 3, 180... 

Fig. 6. a Capillary GC trace of total alkaloid extract of Delphinium occidentale. b Capillary GC trace of total alkaloid extract of D. barbeyi. Peaks 1 Delpheline 2 deltamine 3 deltaline 4 14-acetyldictyocarpine 5,6 dictyocarpine and dictyocarpinine 7 delcosine. The GC conditions are described in Table 5. (Manners and Ralphs 1989)... 

Fig. 13.23 Plot of the first three canonical variables from the discriminant analysis of the tabulated ESI-MS data for toxic Delphinium species according to Ewan [59]. Croups A and B are Delphinium barbeyi, group C is D. occidentale, group D is D. glaucum, and group E is a hybrid (barb, x occi.).

Some Delphinium species are poisonous to cattle and cause losses in livestock on mountain grazing ranges. Manners and Ralphs (1989) report the analysis of the alkaloid content of D. occidentale and D. barbeyi on a medium polarity column. Dictyocarpine and dictypocarpinine could not be separated under the conditions used and quantitative analysis of five alkaloids was shown to be possible. Figure 6 illustrates a GC separation of these compounds. 

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