Deinococcus

Muller D J, Baumeister W and Engel A 1996 Conformational change of the hexagonally packed intermediate layer of Deinococcus radiodurans monitored by atomic force microscopy J. Bactehol. 178 3025... 

Deinococcus radiodurans strain Rl Anaerobic cultures grown with lactate could reduce chromate, although reduction was increased by the presence of electron transfer to AQDS. Reduction of U(VI) and Tc(VII) could also be accomplished in the presence of the electron transfer agent (Fredrickson et al. 2000). 

Michels, D.A., Hu, S., Damhrowitz, K.A., Eggertson, M.J., Lauterhach, K., Dovichi, NJ. (2004). Capillary sieving electrophoresis-micellar electrokinetic chromatography fully automated two-dimensional capillary electrophoresis analysis of Deinococcus radiodurans protein homogenate. Electrophoresis 25, 3098-3105. 

Figure 9.11 Deinococcus radiodurans. (Reproduced by permission ofOak Ridge National Laboratory)...

Actinobacteria -Aquificales — Cya nobacts r a —/ Cytophagales - / Spirochaetales —/ Chlamydiales — Thermotogales — CFB/G een suifu — Green nonsulfur— rhermus/Deinococcus—... 

Diaz, B. and Schulze-Makuch, D. (2006). Microbial survival rates of Escherichia coli and Deinococcus radiodurans under low temperature, low pressure, and UV-Irradiation conditions, and their relevance to possible Martian life. Astrobiology 6, 332-347. 

Examples of especially dry environments on Earth include the Atacama Desert of northern Chile and the Dry Valleys of Antarctica. Dose et al. (2001) exposed spores, conidia, and cells of several microbes to 15 months of desiccation in the dark at two locations of the Atacama Desert. Bacillus subtilis (bacteria) spores (survival 15%) and Aspergillus niger (fungi) conidia (survival 30%) outlived other species. Deinococcus radiodurans (bacteria) did not survive the desert exposure because they were readily killed at RH between 40% and 80%, which occurred during desert nights (Dose et al. 2001). 

Damaging radiation (survival, not growth) 10,000-11,000 grays (gamma radiation), Deinococcus radiodurans German cockroach (Blatella germanica) can survive exposure to radiation above 1,000 grays No natural source of radiation at level tolerated by D. radiodurans... 

Agrobacterium tumefaciens Brucella melitensis Burkholderia fungorum (2) Campylobacter jejuni Caulobacter crescentus Chlorobium tepidum Chloroflexus aurantiacus Deinococcus radiodurans Magnetospirillum magnetotacticum (2) Mesorhizobium loti (2) Methanosarcina acetivorans (2)b Methanosarcina mazei (2)b Mycobacterium tuberculosis Myxococcus xanthus Nostoc punctiforme (2)... 

Thermus aquaticus Thermus thermophilus Thermus filiformis Deinococcus radiodurans Escherichia coli Haemophilus influenzae Streptococus pneumoniae Mycobacterium tuberculosis Mycobacterium laprae Treponema pallidum Chlamydia trachomatis Boriela burgdorferi Helicobacter pyroli Lactococcus lactis Mythelobacterium Rhodothermus obamensis Rickettsia prowazekii Streptomyces coelicolor Bacillus stearothermophilus Synechocystis sp Aquifex aeolicus Apse-1 DNA polymerase T7 DNA polymerase T5 DNA polymerase ... 

X-ray crystal structures of the three 14-metnbered macrolides, erythromydn, clarithromycin and roxithromycin have been solved bound to the Deinococcus radiodu-rans (Dr) large subunit [4]. They bind at about the same location as the 15- and... 

Fig. 1. Dendrogram of relationships among photosynthetic prokaryotes and their relatives, after Stackebrandt and Woese [2], Five bacterial phyla [1] containing photosynthetic members are shown. The exact relationship of Heliobacterium chlorum to the gram-positive bacteria is not yet known. Not shown are the other five phyla without known photosynthetic-members peptidoglycan-less bacteria bacteroids, cytophagas and flavobacteria spirochaetes and leptospiras bdellovibrios, myxococci, and certain S and SOj reducers and Deinococcus. PS = photosynthetic for further explanation, see text.

Another modification of the LC LC-MS approach is the use of ultra-high-efficiency RPLC columns [54-55]. The system consists of a 800x0.32-pm-lD SCX column (packed with 3-pm polysulfoethyl aspartamide-bonded silica), a 40-nunx75-pm-ID RPLC trapping column, and a 800-nunx30-pm-lD RPLC colunm. The system was applied to the identification of proteins in the proteome of Deinococcus radiodurans [54], and of human plasma proteins [55]. In that case, more than 800 proteins, i.e., both low-abundance cytokines and high-abundance proteins, were identified from in total 365 pg plasma. 

Figure 1 (a) Two views of the 5OS from Haloarcula marismortui (I Ml K). (b) Two views of the 5OS from Deinococcus radiodurans (1NWY). (c) Two views of the 305 from Thermus thermophilus (1FJG). (d) Two views of the 70S from T. thermophilus (1VSA). Proteins have been left out for simplicity. 

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