Deinococcus radiodurans proteome

H.R. Venkateswaran, A. Wong, L.K. Zhao, R. Smith, R.D. Global analysis of the Deinococcus radiodurans proteome by accnrate mass tags. Proc. Natl. Acad. Sci. 2002, 99(17), 11049-11054. 

Another modification of the LC LC-MS approach is the use of ultra-high-efficiency RPLC columns [54-55]. The system consists of a 800x0.32-pm-lD SCX column (packed with 3-pm polysulfoethyl aspartamide-bonded silica), a 40-nunx75-pm-ID RPLC trapping column, and a 800-nunx30-pm-lD RPLC colunm. The system was applied to the identification of proteins in the proteome of Deinococcus radiodurans [54], and of human plasma proteins [55]. In that case, more than 800 proteins, i.e., both low-abundance cytokines and high-abundance proteins, were identified from in total 365 pg plasma. 

Stem cells, haploid germ cells, somatic cells, transformed cells. The ancient unicellular life forms survived under the most adverse conditions in a chemically hostile, overheated (boiling water pouring lava volcanic fumes), chemically imbalanced as to excessive acidity or alkalinity, and heavily radioactive environment. The y-rays-irradiated but radiation-resistant Deinococcus radiodurans (Figure 4) suffers genomic damage it is its chaperone proteins in its proteome that perform the rescue of the genome (Mediterranean Instimte for Life Sciences, Split, Croatia). 

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