Dehydrogenase Isoenzymes

Chick J, Lehert P, Landron F, et al Does acamprosate improve reduction of drinking as well as aiding abstinence J Psychopharmacol 17 397-A02, 2003 Chrostek L, Jelski W, Szmitkowski M, et al Gender-related differences in hepatic activity of alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in humans. J Clin Lab Anal 17 93-96, 2003... 

Gay, R. J. McComb, R. B. and Bowers, G. N. Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehyrogenase activity. Clin. Chem. (1968), U, 740-753. 

Rosalki, S. B. Standardization of isoenzyme assays with special reference to lactate dehydrogenase isoenzyme electrophoresis. Clin. Biochem. (1974), 2> 29-40. 

Maly IP, Toranelli M, Sasse D. Distribution of alcohol dehydrogenase isoenzymes in the human liver acinus. Histo-chem Cell Biol 1999 111 391-397. 

Bittar, P. G., Charnay, Y., Pellerin, L. etal. Selective distribution of lactate dehydrogenase isoenzymes in neurons and astrocytes of human brain. /. Cereb. Blood Flow Metab. 16 1079-1089,1996. 

Blood transfusions can also lead to misleading laboratory values. Stored blood bank blood can appreciably increase the blood ammonia levels (PIO). Blood stored 7 days has been found to contain over 1100 /xg/ml. A case has been reported of a patient receiving massive blood transfusions whose serum contained an additional lactic dehydrogenase isoenzyme (a splitting of the LDH-1 band). The authors concluded that the extra band was not an artifact, but rather represented an abnormal H subunit present in one or more of the transfused plasmas (F6). 

KlO. Kreiitzer, H. H., and Fennis, W. H. S., Lactic dehydrogenase isoenzymes in blood serum after storage at different temperatures. Clin. Chim. Ada 9, 64-68 (1964). 

Yeast contains two cytosolic alcohol dehydrogenase isoenzymes.66 Alcohol dehydrogenase I, present in large amounts in cells undergoing fermentation,... 

Tang PT, Wang XP, Lu DS. 1992. Changes in lactate dehydrogenase isoenzymes of mitochondriaand microsomes in hepatic damage caused by phosphorus, sodium arsenite, and carbon tetrachloride. Zhongguo Yaolixue Yu Dulixue Zazhi 6(4) 292-296. 

Variations in the structures of enzymes within a species can also occur between and within ethnic groups of the same species. For example, a number of different alcohol dehydrogenase isoenzymes have been observed in some Asians. 

Jaffe AS, Landt Y, Parvin CA,et al. Comparative sensitivity of cardiac troponin I and lactate dehydrogenase isoenzymes for diagnosing acute myocardial infarction. Clin Chem 42 1770-1776,1996. 

G21. Grant, C. M., Quinn, K. A., and Dawes, I. W., Differential protein S-thiolation of glyceraldehyde-3-phosphate dehydrogenase isoenzymes influences sensitivity to oxidative stress. Mol. Cell Biol. 19, 2650-2656 (1999). 

ST90 Jensen, A.E., Reikvam, A., Nordgird, S., Pedersen, K.G. and Asberg, A. (1990). Diagnostic accuracy of Kodak creatine kinase MB, Stratus creatine kinase MB, and lactate dehydrogenase isoenzyme 1 in serum after acute myocardial infarction. Clin. Chem. 36, 1847-1848. 

Human alcohol dehydrogenase isoenzymes have been divided into three classes. Class I isoenzymes contain a, p, yl, and y2 subunit isoenzymes, are cationic at neutral pH, and are strongly inhibited by substituted pyrazole inhibitors. Class II isoenzymes contain the 7i isoenzyme subunit, are cationic at neutral pH, and are relatively insensitive to pyrazole inhibition. Class III isoenzymes contain the X subunit, are anionic at neutral pH and are insensitive to pyrazole inhibitors. 

Methods for the Separation and Quantification of Lactate Dehydrogenase Isoenzymes... 

Chatterly S, Sun T, Lien Y. Diagnostic value of lactate dehydrogenase isoenzymes in cerebrospinal fluid. J Clin Lab Anal 1991 5 168-74. 

Moses GC, Ross ML, Henderson AR. Ten electrophoretic methods compared with a selected method for quantifying lactate dehydrogenase isoenzymes in serum. Clin Chem 1988 34 1885-90. 

Von Eyben FE, A systematic review of lactate dehydrogenase isoenzyme 1 and germ ceU tumors. Clin Biochem 2001 34 441-54. 

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