Databases rapidly expanding

In parallel with these advances in mRNA detection and assessment, significant advances in the detection, characterization, and quantitation of proteins have been made. This is not only a result of the rapidly expanding database of protein sequences but also of the extraordinary advances in sensitivity of mass spectrometry techniques. It is proposed, with some real sense of expected success, that protein characterization at the whole genome level will be forthcoming (Domon and Aebersold, 2006). 

Since the literature about CDs is rapidly expanding ( 45,000 references in the CAS database from September 2008), this review is focused on stating basic principles exemplified by original literature. Older literature about CDs has already been summarized in several review articles dealing with CDs in general [22,23], CD crystallography [24,25], CD derivatives [26], stabilities of CD ICs [27], CD rotax-anes [28], and CD polyrotaxanes [29-31],... 

Bioinformatics is a rapidly expanding field, involving the application of computer technology to the management of biological information. Here, only two of the key bioinformatics components related to biomolecular engineering, database and computer modeling, are discussed. 

A searchable, relational database was first developed by Mats Alffedsson and Sandberg Analytical Data AB in Sweden to enable rapid identification of suitable reference materials for element analysis in metallmrgical samples. Since then the database has been expanded and it is now supplied with information about more than II 000 RMs and CRMs. The database also allows continuous addition of RMs of interest to the user. 

Webster s definition for database is a large collection of data in a computer, organized so that it can be expanded, updated, and retrieved rapidly for various uses. An LC/MS database established for drug impurities contains multi-dimensional information such as relative retention times, UV spectra, molecular mass and substructural information. In order for the information to be updated and expanded, the methods used for information collection need to be unified. A generic LC/MS method allows relevant information to be collected in a consistent... 

Due to the limitations of prediction, there is a need for projects that will collect data on subcellular location for a variety of conditions. These projects determine protein location rather than predict it. Although these projects are useful in their own right, they also serve as a way to expand the database for the prediction of protein location. One powerful approach to determination is to isolate specific organelles or stmctures and then identify their components by expression proteomics methods (Bmnet et al. 2003). This provides information rapidly for large numbers of proteins but has limited ability to distinguish different domains within an organelle. This chapter will focus on a complementary approach, automated determination of subcellular location using fluorescence microscopy. 

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