Culture hormonal effects

Clearly, major differences seem suggested in the mechanisms of hormone-membrane receptor interactions leading to various post-binding events [11], For FSH, as an example, there have been reports of hormone effects on membrane potentials of cultured Sertoli cells [12] and on conductance of artificial lipid membranes [13], as well as on amino acid transport systems [14]. Indeed, there has been one report of receptor-mediated gonadotropin action without receptor occupancy... 

G20. Griffin, M. J., and Cox, R. P., Studies on the mechanism of hormonal induction of alkaline phosphatase in human cell cultures. I. Effects of Puromycin and Actinomycin D. J. Cell Biol. 29, 1-9 (1966). 

De Vellis J, McGinnis JF, Breen GAM, Leveille P, Bennett K, McCarthy K (1977) Hormonal effects on differentiation in neural cultures. In Federoff S, Hertz L (Eds), Cell, Tissue and Organ Cultures in Neurobiology, Academic Press, New York, 485-514. 

Auxins and cytokinins are the most widely studied plant hormones on account of their importance for cell growth and division and, from a practical point of view, for their profound impact on organogenesis when plant cells and tissues are cultured in vitro. Not surprisingly, therefore, both these hormones were included in pioneering studies of hormonal effects on the orientation of MTs in relation to cell growth (e.g. references... 

The outcome seemed due to a particular effect of molybdenum exposure rather than to secondary copper deficiency. The reduction in peak levels of ovulatory luteinizing hormone suggested that events controlling estrus and sexual interest in males had been altered by molybdenum. These effects were the consequence of an inhibitory influence of molybdenum on estrogen and androgen receptor activity that had been demonstrated in cell culture. The effects of sodium molybdate have been described for estrogen receptors (Joab etal. 

There are only a few other papers on hormonal effects on sieve-tube regeneration aside from those on cultured material. Benayoun et al. (1975) reported that lAA could replace distal leaves of Cucumis or Coleus in causing sieve-tube regeneration but provided no data on the point. 

MOUSAVi Y, ADLERCREUTZ H (1993) Genisteiu is an effective stimulator of sex hormone-binding globulin production in hepatocarcinoma hinnan hver cancer cells and suppresses proliferation of these cells in culture. Steroids. 58 301-4. 

Zearalenone was also found to be more effective than cytokinin treatment in inducing shoots in in vitro winter wheat production. Moreover, both zearalenone and cytokinins increased the activity of antioxidant enzymes in wheat callus undergoing regeneration, and it is very likely that they also stimulated the plant regeneration process (Szechynska-Hebda et al. 2007). The effectiveness of regeneration on media containing zearalenone shows the possibility of using zearalenone as an alternative hormone also to cytokinins in winter wheat callus culture. 

Surfactants were tested at concentrations ranging from 0.1 mM to 10 nM dissolved in the culture medium, with the concentration of solvent always maintained below 0.1%. Results are expressed as means SD. In the proliferation yield experiments, each point represents the mean of three counts from the four culture wells. Mean cell numbers were normalised to the control, equal to 1, to correct for differences in the initial plating density. The proliferative effect (PE) was expressed as the ratio between the highest cell yield obtained with each chemical tested and the hormone-free control. Proliferation experiments were repeated at least three times. 

A role for a collagenase, presumably fibroblast-type collagenase, in bone resorption has been indicated by studies employing the Searle A-carboxyl alkyl synthetic collagenase inhibitor CI-1 (compound (197) in Table 8.18) and its less potent stereoisomer CI-2 (compound (198) in Table 8.18) [207]. Cultured embryonic mouse calvaria treated with parathyroid hormone exhibit loss of calcium and show pronounced collagen resorption. CI-1 inhibited the collagen resorption in a dose-dependent manner at significantly lower concentrations than CI-2, but had only a small effect on calcium loss. This inhibitory effect was reversible and not due to inhibitor cytotoxicity. 

Williams J, Foster PM. 1989. The effects of 1,3-dinitrobenzene and mono-(2-ethylhexyl) phthalate on hormonally stimulated lactate and pyruvate production by rat Sertoli cell cultures. Toxicol Lett 47 249-257. 

Yamaguchi, M. and Kishi, S. (1995b). Inhibitory effect of zinc-chelating dipeptide on parathyroid hormone-stimulated osteoclast-like cell formation in mouse marrow cultures Involvement of calcium signalling. Peptides 16, 629-633. 

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