Collagenases fibroblast-type

A role for a collagenase, presumably fibroblast-type collagenase, in bone resorption has been indicated by studies employing the Searle A-carboxyl alkyl synthetic collagenase inhibitor CI-1 (compound (197) in Table 8.18) and its less potent stereoisomer CI-2 (compound (198) in Table 8.18) [207]. Cultured embryonic mouse calvaria treated with parathyroid hormone exhibit loss of calcium and show pronounced collagen resorption. CI-1 inhibited the collagen resorption in a dose-dependent manner at significantly lower concentrations than CI-2, but had only a small effect on calcium loss. This inhibitory effect was reversible and not due to inhibitor cytotoxicity. 

Human fibroblast Collagenase (HFC) 57.000 (glycosylated) 52.000 0.32 Type 1, II, III, VII and X collagens gelatins a-Ms casein fibroblasts keratinocytes macrophages U937 cells endothelial cells [27,34]... 

Wilhelm, S. M., Collier, I. E. Manner, B. L., Eisen, A. Z., Grant, G. A. and Goldberg, G. I. (1989). SV-40-transformed human lung fibroblasts secrete a 92-kDa type IV collagenase which is identical to that secreted by normal human macrophages. J. Biol. Chem. 264(29), 17213-17221. 

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