Immunofluorescence imaging

Gonczy This is very difficult to quantitate. As you have seen from the immunofluorescence images, there are many microtubules on either side in wild-type moreover, anaphase B takes places within a couple of minutes. Therefore, it will be difficult to uncover potential transient changes in microtubule numbers using fixed specimens. However, we have generated a green fluorescent protein... 

The patterns of antigen distribution in different organelles are quite characteristic in cultured cells, and examples of such patterns have been published in many journal articles and books (e.g., ref. 2). From immunofluorescence images, antigens restricted to intracellular membranous organelles, cytoskeletal elements, cytosolic compartments, and nuclear... 

Fig. 20.4. STORM imaging of microtubules in a mammalian cell, (a) Conventional immunofluorescence image of microtubules in a large area of a BS-C-1 cell, (b) STORM image of the same area, (c) Conventional and (d) STORM images corresponding to the boxed regions in (a)...

Fig. 20.8. 3D STORM imaging of clathrin-coated pits in a cell, (a) Conventional immunofluorescence image of clathrin in a region of a BS-C-1 cell, (b) The STORM image of the same area with all localizations at different z positions stacked, (c) An x — y cross-section (50-nm thick in z) of the same area, (d, e) Magnified view of two nearby coated pits in 2D STORM (d) and their x — y cross-section in the 3D image (e). (f—h) Serial x — y cross-sections (each 50-nm thick in z) (f) and x — z cross-sections (each 50-nm thick in y) (g) of a CCP, and an x — j/ and x — z cross-section presented in 3D perspective (h)...

Fig. 12 (a-c) Immunofluorescent images of cells in flower and star shapes stained for F-actm (green), vinculin (red) and nuclei (blue), (d) Immunofluorescent images of cells in flower and star shapes stained for myosin Ila. (e) Fluorescent heatmaps of cells stained for myosin Ila as a quantitative measure of contractility. Scale bar. 20 pm [113]... 

Fig. 2 Schematic of liposome loaded with a tri-fusion reporter gene (TF). (A) Schematic representation of the tri-fusion reporter gene (TF) containing Renila luciferase (Rluc), red fluorescent protein (RFP) and HSV-ttk driven by a CMV promoter. (B) Schematic representation of liposome nanoparticle and liposome-DNA coupling reaction. Therapeutic liposomes were loaded with Rluc-RFP-ttk gene. (C) The expression of TF transgene was tracked and analyzed by imaging and immunofluorescence. Imaging of Renilla luciferase intensity from one representative BALB/c mouse [39].

Fig. 3 Actin cytoskeleton and focal adhesion. F-actins were visualized using rhodamine phalloidin (red), and vinculins found in focal adhesions were stained using fluorescently conjugated antibodies. (A) Immunofluorescent images of a thin filamentous actin meshwork and vinculins showed that osteoblasts contain fewer and smaller focal adhesions. (B) In contrast, hMSCs showed thick actin stress fibers, and multiple and large adhesion contacts.

Confocal immunofluorescent images of tracheal cells using the anti-Chc (red) and the anti a-Sjjectiin (green) and anti-Crb (green) antibodies. The a-Sp>ectrin marks cell membranes and Crb indicates apical cell membranes. (A) In stage 17 wild-type embryos Che (red) is distributed towards the apical cell membrane. (B,C) In stage 17 f/G4-driven UAS-RNAi-erF knock-down embryos and crb null mutant embryos, Che showed intracellular mis-localization in tracheal cells (arrows). (D) Tracheal Crb overexpression led to intensive Che co-localization with Crb (arrows). Scale bars=10 im. 

Neurons do not attach, mature, or survive properly when grown directly on plastic tissue culture dishes. In addition, immunofluorescence imaging and quantification can only be carried out easily on cells grown on cover-slips. Therefore, we plate hippocampal neurons onto glass coverslips that have been cleaned, sterilized, and coated with poly-l-lysine to improved cell adhesion (Banker, 1991). 

Figure 10.6 Immunofluorescence images of mesenchymal stem cells (MSCs) cultured on (a) nanofibrous poly(E-caprolactone) scaffolds and (b) tissue culture polystyrene plate showing different cytoskeletal organization (actin and nucleus were stained with Phalloidin and 4, 6-diamidmo-2-phenylindole [DAPI]).

Fig. 8. Immunofluorescence images of HEPM cells attached to PPCs after 1 and 3 days (reproduced...

FIGURE 37.1 Phase contrast image (a) and OCT4 immunofluorescence image (b) of an HI hESC colony cocul-... 

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