Cooled charge-coupled device

Jackson, P., The use of polyacrylamide-gel electrophoresis for the high-reso-lution separation of reducing saccharides labeled with the fluorophore 8-ami-nonaphthalene-l,3,6-trisulfonic acid. Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device, Biochem. ]., 270, 705, 1990. 

Using the same PAbs an optical biosensor system has been developed for 2,4,6-TCP [224]. The principle is the detection of laser-induced fluorescence (LIF) in single microdroplets by a homogeneous quenching fluorescence immunoassay (QFIA). The competitive immunoassay occurs in microdroplets (d=58.4 mm) produced by a piezoelectric generator system. A continuous Ar ion laser (488 nm) excites the fluorescent tracer and its fluorescence is detected by a spectrometer attached to a cooled, charge-coupled device (CCD) camera... 

The use of a sensitive camera system, such as a cooled charged-coupled device (CCD) camera, facilitates resolution and recording of the image, including the pseudocolors generated by probes 4-7. 

Super-cooled charge-coupled device (CCD)-based imagers such as the Perkin Elmer ViewLux or the General Electric LeadSeeker collect all data on the wells simultaneously through a CCD and work like digital cameras. The readers, although expensive, tend to be faster, but less flexible. 

Urwin, V. E. and Jackson, R. (1993) Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to the firstdimensional isoelectric focusing imaging of the fluorescent protein spot patterns using a cooled charge-coupled device. Anal. Biochem., 209, 57-62. 

Start timelapse imaging. We use a scientific-grade cooled charged-coupled device (CCD camera) on a multiwavelength MetaMorph... 

D. Cooled Charge-Coupled Device Cameras Successful Approaches to Nuclear Structure... 

The Raman spectrometer is equipped with a Peltier-cooled charge-coupled device (CCD) detector, together with a laser which can be selected depending on the application. The 25-mW air-cooled argon ion laser (Spectra Physics) lasing at 514 nm is the most versatile for gemstone work. 

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