Cooled charge-coupled device camera

D. Cooled Charge-Coupled Device Cameras Successful Approaches to Nuclear Structure... 

Using the same PAbs an optical biosensor system has been developed for 2,4,6-TCP [224]. The principle is the detection of laser-induced fluorescence (LIF) in single microdroplets by a homogeneous quenching fluorescence immunoassay (QFIA). The competitive immunoassay occurs in microdroplets (d=58.4 mm) produced by a piezoelectric generator system. A continuous Ar ion laser (488 nm) excites the fluorescent tracer and its fluorescence is detected by a spectrometer attached to a cooled, charge-coupled device (CCD) camera... 

The use of a sensitive camera system, such as a cooled charged-coupled device (CCD) camera, facilitates resolution and recording of the image, including the pseudocolors generated by probes 4-7. 

Super-cooled charge-coupled device (CCD)-based imagers such as the Perkin Elmer ViewLux or the General Electric LeadSeeker collect all data on the wells simultaneously through a CCD and work like digital cameras. The readers, although expensive, tend to be faster, but less flexible. 

Start timelapse imaging. We use a scientific-grade cooled charged-coupled device (CCD camera) on a multiwavelength MetaMorph... 

The imaging hardware needs to be sensitive enough to detect minimally labeled proteins. Because a cooled, coupled charge device (CCD) had been used previously to image fluorescently labeled proteins in the gel, we decided to use a scientific grade CCD camera. CCD cameras without cooling do not have the requisite sensitivity of low noise capabilities. 

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