Charge-coupled devices cooling

Jackson, P., The use of polyacrylamide-gel electrophoresis for the high-reso-lution separation of reducing saccharides labeled with the fluorophore 8-ami-nonaphthalene-l,3,6-trisulfonic acid. Detection of picomolar quantities by an imaging system based on a cooled charge-coupled device, Biochem. ]., 270, 705, 1990. 

Using the same PAbs an optical biosensor system has been developed for 2,4,6-TCP [224]. The principle is the detection of laser-induced fluorescence (LIF) in single microdroplets by a homogeneous quenching fluorescence immunoassay (QFIA). The competitive immunoassay occurs in microdroplets (d=58.4 mm) produced by a piezoelectric generator system. A continuous Ar ion laser (488 nm) excites the fluorescent tracer and its fluorescence is detected by a spectrometer attached to a cooled, charge-coupled device (CCD) camera... 

Except for in house preliminary studies, the intensities of X-rays diffracted by hydrogenase crystals are now usually obtained with synchrotron radiation (Fig. 6.2) and detected by image plate or charge coupled device (CCD) detectors. To limit the damage induced by the powerful photon flux of synchrotrons, the crystals are usually mounted in a small loop, flash cooled in either liquid propane or nitrogen and stored... 

The use of a sensitive camera system, such as a cooled charged-coupled device (CCD) camera, facilitates resolution and recording of the image, including the pseudocolors generated by probes 4-7. 

Super-cooled charge-coupled device (CCD)-based imagers such as the Perkin Elmer ViewLux or the General Electric LeadSeeker collect all data on the wells simultaneously through a CCD and work like digital cameras. The readers, although expensive, tend to be faster, but less flexible. 

Urwin, V. E. and Jackson, R. (1993) Two-dimensional polyacrylamide gel electrophoresis of proteins labeled with the fluorophore monobromobimane prior to the firstdimensional isoelectric focusing imaging of the fluorescent protein spot patterns using a cooled charge-coupled device. Anal. Biochem., 209, 57-62. 

Start timelapse imaging. We use a scientific-grade cooled charged-coupled device (CCD camera) on a multiwavelength MetaMorph... 

D. Cooled Charge-Coupled Device Cameras Successful Approaches to Nuclear Structure... 

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