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Cloning system Gateway

ORFs are transferred to an expression vector without the requirement of amphfica-tion by PCR or subsequent concern for PCR-induced mutations. Gateway is an example of a new genre of recombination-based cloning systems that fadhtate subcloning of genetic material by site-spedfic recombination and enable transfer and assembly of large numbers of DNA elements using a standard mechanism. [Pg.607]

The ability efficiently to manipulate DNA elements afforded by the Gateway cloning system has opened (see also Part III, Chapters 1 and 7) - and wiU continue to open - many doors to novel experimental protocols that wiU be invaluable in basic research finked with the development of modem biopharmaceuticals. [Pg.619]

Gateway recombinatorial cloning system 606 Gateway vector system 611 Gaucher s disease 8... [Pg.1857]

The bnilding block can also be cloned by traditional ligation provided that restriction sites are added on the outermost primers, or by cloning systems such as Gateway that has recombination sites added to the outmost primers. If a uracil-specific excision reagent (USER) fusion system is used, it is important to note that wild-type archaeal DNA polymerases are inhibited by the deoxyuracil. [Pg.9]

Gateway cloning technology is a modification of the recombination system of phage k (Walhout et ah, 2000 Hartley et ah, 2000). The Gateway system utilizes a minimum set of components of the k system for in vitro transfer of DNA, namely the k integrase protein, k excisionase, the E. coli protein... [Pg.24]

Systematic Cloning of an ORFeome Using the Gateway System... [Pg.11]

Key words ORFeome, The Gateway system, Cloning, High-throughput, Schizosaccharomycespombe... [Pg.11]

Learning from Viruses High-throughput Cloning using the Gateway System to Transfer Genes without Restriction Enzymes... [Pg.605]

See other pages where Cloning system Gateway is mentioned: [Pg.4]    [Pg.6]    [Pg.7]    [Pg.7]    [Pg.8]    [Pg.9]    [Pg.9]    [Pg.10]    [Pg.11]    [Pg.12]    [Pg.54]    [Pg.605]    [Pg.614]    [Pg.615]    [Pg.615]    [Pg.1996]    [Pg.1998]    [Pg.39]    [Pg.241]    [Pg.24]    [Pg.24]    [Pg.13]    [Pg.13]    [Pg.15]    [Pg.167]    [Pg.705]    [Pg.360]    [Pg.150]    [Pg.307]    [Pg.605]    [Pg.606]    [Pg.606]    [Pg.607]    [Pg.607]    [Pg.608]    [Pg.610]   
See also in sourсe #XX -- [ Pg.5 , Pg.6 , Pg.7 , Pg.8 , Pg.12 ]



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Gateway cloning

Learning from Viruses High-throughput Cloning using the Gateway System to Transfer Genes without Restriction Enzymes

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