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Cloning system

Bolivar, F., Rodriguez, R. L., Greene, P. J., et al., 1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2 95-113. A paper describing one of the early plasmid cloning systems. [Pg.423]

The topoisomerase and Cre-fox-based cloning systems described above may not be ideal for all experimental situations. However, alternative... [Pg.40]

Crameri, R., and Suter, M. (1993). Display of biologically active proteins on the surface of filamentous phages a cDNA cloning system for selection of functional gene products linked to the genetic information responsible for their production. Gene 137, 69-75. [Pg.112]

Similar approaches to cloning of bacterial xylanases have been used for genes from Clostridium acetobutylicum (30), Bacillus polymyxa (31), Bacteroides succinogenes (32), Clostridium thermocellum (33) and Pseudomonas fluorescens subsp. cellulosa (34). In each case the xylanases were predominantly located intracellularly and the levels of xylanases produced from cloned systems were, in general, very low in comparison to yeast and fungal systems. A comparison of the production yields and extent of extracellular production for various cloned xylanase genes is found in Table... [Pg.643]

I. Unlike most other cloned systems, the xylanase preparation from B. succinogenes was contaminated with cellulase activity. [Pg.643]

The preparation of xylanases from cloned systems has permitted production of cellulase-free xylanases. The xylanases produced from cloned... [Pg.648]

ORFs compatible with the Flexi cloning system can be easily ttansfetted between exptession vectots with diffetent antibiotics (ampicillin-tesistant and kanamycin-tesistant vectots) (tttNote 2)... [Pg.29]

Blommel PG, Martin PA, Wrobel RL, Steffen E, Fox BG. (2006) Hi efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system Protein Expr Purif 47, 562-570. [Pg.40]

For many years most cloning was done in E. coli, but cloning systems have now been developed for many other bacteria including Bacillus and other grampositive bacteria, and also for yeast, insect cells, animals, and plants.133-135... [Pg.1497]

Cloning Systems A Laboratory Manual, Cold Spring Harbor Lab. Press, Cold Spring Harbor, New York... [Pg.1521]

Despite the success and broad applications of E. coli cloning systems, instances occur in which gene products cannot be made in this bacterium. Either they are not synthesized in... [Pg.688]

Effective cloning systems are available for a variety of bacterial hosts, including Bacillus subtilis, Streptomyces spp., and Agrobacter tumefaciens. Cloning systems have also been developed for eukaryotic hosts such as the yeast Saccharomyces cerevisiae, mammalian cells in tissue culture, and plant cells. [Pg.689]

See other pages where Cloning system is mentioned: [Pg.460]    [Pg.461]    [Pg.36]    [Pg.38]    [Pg.41]    [Pg.42]    [Pg.43]    [Pg.46]    [Pg.262]    [Pg.551]    [Pg.582]    [Pg.350]    [Pg.351]    [Pg.642]    [Pg.643]    [Pg.643]    [Pg.644]    [Pg.59]    [Pg.6]    [Pg.6]    [Pg.7]    [Pg.7]    [Pg.8]    [Pg.9]    [Pg.9]    [Pg.10]    [Pg.11]    [Pg.12]    [Pg.25]    [Pg.26]    [Pg.224]   
See also in sourсe #XX -- [ Pg.461 , Pg.462 ]



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