Circular dichroism spectroscopy nucleic acids

See also Biochemical Applications of Raman Spectroscopy Biomacromolecular Applications of Circular Dichroism and ORD Carbohydrates Studied by NMR Circularly Polarized Luminescence and Fluorescence Detected Circular Dichroism Induced Circular Dichroism Magnetic Circular Dichroism, Theory Nucleic Acids and Nucleotides Studied Using Mass Spectrometry Organometallics Studied Using Mass Spectrometry Polymer Applications of IR and Raman Spectroscopy Proteins Studied Using NMR Spectroscopy Vibrational CD Spectrometers Vibrational CD, Theory. [Pg.1244]

Optical Spectroscopy General principles and overview, 246, 13 absorption and circular dichroism spectroscopy of nucleic acid duplexes and triplexes, 246, 19 circular dichroism, 246, 34 bioinorganic spectroscopy, 246, 71 magnetic circular dichroism, 246, 110 low-temperature spectroscopy, 246, 131 rapid-scanning ultraviolet/visible spectroscopy applied in stopped-flow studies, 246, 168 transient absorption spectroscopy in the study of processes and dynamics in biology, 246, 201 hole burning spectroscopy and physics of proteins, 246, 226 ultraviolet/visible spectroelectrochemistry of redox proteins, 246, 701 diode array detection in liquid chromatography, 246, 749. [Pg.6]

Several direct methods are available to analyze the tertiary structure of ODNs like nuclear magnetic resonance (NMR) and X-ray crystallographic (XRC) techniques, which needs a sophisticated setup and infrastructure. An alternative but indirect method to study the structure and conformations of nucleic acids is circular dichroism spectroscopy (CD spectroscopy) (25, 26), where circular dichroism refers to the differential absorption of left and right circularly polarized light (27). [Pg.48]

Bioactive macromolecules like peptides, proteins, and nucleic acids have been successfully embedded in planar LbL films. An important question is the retention of the bioactivity of the film-embedded biomolecules. The structural properties and stability of the LbL films formed from synthesized polypeptides of various amino acid sequences were recently reported [50]. The authors showed that control over the amino acid sequence enables control over non-covalent interpolypeptide interaction in the film, which determines the film properties. Haynie and coworkers showed by circular dichroism spectroscopy that the extent of adsorption of poly(L-glutamic acid) (PGA) and poly(L-lysine) (PLL) in the LbL films scales with the extent of secondary structure of the polypeptides in solution [51]. Boulmedais demonstrated that the secondary structure of the film composed of these polypeptides is the same as the peptide structure in the complex formed in solution [52], as found by Fourier transform IR spectroscopy (FUR). [Pg.138]

Absorption and Circular Dichroism Spectroscopy of Nucleic Acid Duplexes and Triplexes... [Pg.19]

In recent years, circular dichroism spectroscopy has been widely applied in investigations concerning the molecular structure of chiral polymers. It is a powerful tool for revealing the secondary structures of biological macromolecules, for instance of polypeptides, proteins, and nucleic acids in solution. An... [Pg.25]

Combined SAXS/Circular dichroism beamline. Biological macromolecules, such as proteins, carbohydrates and nucleic acids, are composed of many optically active or chiral units that exhibit large Circular Dichroism (CD) signals. CD spectroscopy has therefore been used extensively in the study of proteins, where asymmetric carbon atoms in their amino acid backbone give rise to a CD spectrum. The shape of the spectrum depends on the protein s secondary structure content and allows the proportions of helix, beta structure, turns and random to be determined. [Pg.271]

Electronic circular dichroism (CD) spectroscopy of nucleic acids... [Pg.1]

Circular Dichroism Vibrational Configuration Interaction Configuration Interaction PCI-X and Applications Density Functional Theory (DFT), Hartree-Fock (HF), and the Self-consistent Field Magnetic Circular Dichroism of n Systems Molecular Magnetic Properties Nucleic Acid Conformation and Flexibility Modeling Using Molecular Mechanics Spectroscopy Computational Methods Stereochemistry Representation and Manipulation. [Pg.380]

See also Biomacromolecular Applications of Circular Dichroism and ORD Chiroptical Spectroscopy, Emission Theory Chiroptical Spectroscopy, Oriented Molecules and Anisotropic Systems Chiroptical Spectroscopy, General Theory Macromolecule-Ligand Interactions Studied By NMR Nucleic Acids and Nucleotides Studied Using Mass Spectrometry Nucleic Acids Studied Using NMR Pharmaceutical Applications of Atomic Spectroscopy. [Pg.874]

Circular dichroism (CD) spectroscopy is a form of absorption spectroscopy that is based on measuring the difference in the absorbance by a substance of right- or left-circularly polarized light. CD is exhibited by chiral molecules, and represents a powerful tool for revealing the structures of biological macromolecules such as polypeptides, proteins and nucleic acids in solution. In the case of proteins, CD spectroscopy not only allows the discrimination between the different structural types, such as a-helix, parallel and antiparallel (3-pleated sheets and (3-turns, but also permits an estimation of the relative contents of these structures. This is... [Pg.176]

Circular dichroism is largely employed to investigate interactions among different biomolecules (protein-protein, protein-nucleic acid, etc.), and among biomolecules and other ligands (for examples, molecules of medicinal interest). Also in this case, not only thermodynamic (association constants, reaction enthalpy and entropy) and kinetic (reaction rates and activation barriers) parameters can be determined, but also different association modes (e.g., DNA intercalation or association in the minor or major groove of the double helix) can be distinguished. Finally, CD spectroscopy is applied in analytical sciences to determine the purity of optically active compounds. [Pg.145]

Other methods for studying nucleic acids and their complexes include infrared spectroscopy. By use of surface-enhanced infrared absorption spectroscopy, in situ hybridisation kinetics have been measured,and detection of formation of G-quadruplexes has been studied using a combination of IR and vibrational circular dichroism. ° ... [Pg.200]