Nucleic acids, conformation

Mathematical Model of the Nucleic Acids Conformational Transitions with Hysteresis over Hydration-Dehydration Cycle... 

Medicinal Chemistry. IV. American Chemical Society. Meeting (186th 1983 Washington, D.C.) V. Series. [DNLM I. Nucleic acid conformation—Congresses. 

The biological functions of the nucleic acids involve the participation of metal ions. In particular K+ and Mgr+ stabilize the active nucleic acid conformations. Mg2+ also activates enzymes which are involved in phosphate transfer reactions and in nucleotide transfer. The monomeric nucleotides are also involved in a number of metabolic processes and here again metal ions are implicated. Consequently, there has been considerable attention focussed on the coordination properties of these molecules as a means of understanding the mechanism of the metal ion involvements. A number of reviews are available which cover the studies on metal interactions.113 117... 

Cekan, P., et al. (2008). Rigid spin-labeled nucleoside Q A nonperturbing EPR probe of nucleic acid conformation. Nucleic Acids Res. 36, 5946—5954. 

W. F. van Gunsteren, R. Kaptein, and E. R. P. Zuiderweg, in Proceedings of the NATO/ CECAM Workshop on Nucleic Acid Conformation and Dynamics, W. K. Olsen, Ed., Centre de Calcul Atomique et Moleculaire, Orsay, 1984, pp. 79-82. Use of Molecular Dynamics Computer Simulations When Determining Protein Structure by 2D-NMR. 

The "rigid nucleotide as building block in the nucleic adds. In this discussion, which is primarily concerned with aspects of hydrogen bonding, the simplified rigid nucleotide concept will be sufficient in the description of nucleotide and nucleic acid conformation. Accord- ing to this concept [545], there are two basic nucleotide units which differ mainly in sugar pucker ... 

TVo POP, Barrett JC, Kan LD, Miller PS (1972) Proton magnetic resonance studies of nucleic acid conformation. Annu Rev NY Acad Sci 222 290- 306... 

Transverse Relaxation Optimized Spectroscopy (TROSY). - TROSY and CRINEPT are new techniques for solution NMR studies of molecular and supramolecular structures. They allow the collection of high-resolution spectra of structures with molecular weights >100 kDa, significantly extending the range of macromolecular systems that can be studied by NMR in solution. TROSY has already been used to map protein-protein interfaces, to conduct structural studies on membrane proteins and to study nucleic acid conformations in multimolecular assemblies. A number of reviews have been published this year that cover the TROSY technique since its inception. ... 

There has been considerable interest in recent years in the formation of condensed films of purine and pyrimidine bases at the solid-liquid interface. It is well recognised that non-covalent affinities between base pairs play a prevalent role in determining nucleic acid conformation and functionality. Likewise, there has been interest in the role of substrate and non-covalent intermolecular interactions in the configuration of ordered monolayers of purine and pyrimidine bases. There is also more general interest in the interaction of bases with metal surfaces and metal complexes. In the latter case it is noted that the biological role of nucleic acids and certain nucleotides are dependent on metal ions, particularly Mg, Ca, Zn, Mn, Cu and Ni. " Also certain metal complexes, notably of platinum, have the anti-tumour activity, which is linked to their ability to bind to bases on DNA. On a different note, the possibility that purine-pyrimidine arrays assembled on naturally occurring mineral surfaces might act as possible templates for biomolecular assembly has been discussed by Sowerby et al. 

Nucleic acid-bound molecules (ligands, drugs) show marked changes in absorbance and fluorescence as compared to those in free solution. Ethidium bromide (E Br) is the most commonly used indicator for nucleic acid conformational... 

V. J. Hruby in "Drug Design Based on Peptide and Nucleic Acid Conformational Structure," J. Vida, Ed., ACS Monograph Series, 1984, in press. 

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