Chromatography high-performance liquid chromatograph

AD Cooper, GWF Stubbings, M Kelly, JA Tarbin, WHH Farrington, G Shearer. Improved method for the online metal chelate affinity chromatography high-performance liquid chromatographic determination of tetracycline antibiotics in animal products. J Chromatogr A 812 321-326, 1998. 

Isoproturon, dichloroprop-P. bifenox Solvent Size exclusion chromatography High-performance liquid chromatograph [77]... 

Coupling of analytical techniques (detectors) to high-performance liquid chromatographic (HPLC) systems has increased in the last tree decades. Initially, gas chromatography was coupled to mass spectrometry (MS), then to infrai ed (IR) spectroscopy. Following the main interest was to hyphenate analytical techniques to HPLC. 

Reversed-phase high-performance liquid chromatography (HPLC) column 50 mm x 3.2-mm i.d. with Kromasil 5- um Gig packing High-performance liquid chromatograph coupled to a triple-quadrupole mass spectrometer with an atmospheric pressure chemical ionization (APCI) source Gel permeation chromatograph with a 60 mm x 25-mm i.d. column packed with Bio-Beads SX-3 (50-g)... 

Lau, S. Y. M., Taneja, A. K., and Hodges, R. S., Effects of high-performance liquid chromatographic solvents and hydrophobic matrices on the secondary and quaternary structure of a model protein. Reversed-phase and size exclusion high-performance liquid chromatography, /. Chromatogr., 317, 129, 1984. 

N. A. Parris, Instrumental Liquid Chromatography, a Practical Manual on High-Performance Liquid Chromatographic Methods (Journal of Chromatography Library, Vol. 27), Elsevier, Amsterdam, 2nd revised ed., 1984 J. Drozd, Chemical Derivatization in Gas Chromatography (Journal of Chromatography Library, Vol. 19), Elsevier, Amsterdam, 1981 J. F. Lawrence and... 

Hurst, W.J., Martin, R.A. Jr, Tarka, S.M. Jr. and Hall, G.D., Authentication of cocoa in maya vessels using high performance liquid chromatographic techniques, J. Chromatography, 466,279,1989. 

A brief description is given of the way in which modern liquid chromatography has been developed from classical techniques. The important components of a high performance liquid chromatograph are introduced and the method is compared with gas chromatography as a separation technique. 

Sternson et al. [58] used a high performance liquid chromatographic method for the analysis of miconazole in plasma. Miconazole was extracted from alkalinized plasma with n-heptane-isamyl alcohol (98.5 1.5) and separated by high performance performance liquid chromatography on p-Bondapak Ci8 with ultraviolet detection at 254 nm. The mobile phase was methanol-tetrahydrofuran-acetate buffer (pH 5) (62.5 5 32.5) containing 5 mmol octanesulfonate per liter. The flow rate was 2 mL/min. Recovery was 100%. The relative standard deviation for injection-to-injection reproducibility was 0.4% and that for sample-to-sample variation was 5% at high miconazole concentrations (30 pg/mL) and 1% at low (1 pg/mL) concentrations. The limit of detection was 250 ng/mL. 

Fan used a high performance liquid chromatographic method for the qualitative and quantitative analysis of miconazole [59], Miconazole sample was dissolved in methanol and determined by high performance liquid chromatography using methanol-water (75 25) as the mobile phase and ultraviolet detection at 214 nm, the recovery was more than 99.4% and the accuracy was satisfactory for the qualitative and quantitative analysis. 

Katori et al [84] used a high performance liquid chromatographic method for the determination of primaquine phosphate tablets. Chromatography on a TSK Gel-4 (octadecylsilanized) column was used to determine the active ingredient in tablets and injections for treating tropical diseases. The mobile phase used is 26% acetonitrile in 0.05-M pH-3 phosphate buffer and the drug was detected at 260 nm. The peak area and peak height reproducibilities were <1.69%, and results compared well with those of other methods. 

I. Molnar Perl, Advances in the High Performance Liquid Chromatographic Determination of Phenylthiocarbamyl Amino Acids, Journal of Chromatography, A, 661, 43 50 (1994). 

P. Novotna, V. Pacakova, Z. Bosakova and K. Stulik, High performance liquid chromatographic determination of some anthraquinone and naphthoquinone dyes occurring in historical textiles, Journal of Chromatography, A, 863, 235 241 (1999). 

High-performance liquid chromatographic separation with electrochemical detection may provide the best sensitivity for phenol quantification in biological samples. The use of gas chromatography with a flame ionization detector may be a more versatile method, if other non-ionic pollutants must be quantified. The advantages and disadvantages of different methods available for the quantification of phenol and metabolites in biological and environmental samples have been discussed by Tesarova and Packova(1983). 

Kagan M, Chlenov M, Kraml CM. 2004. Normal-phase high-performance liquid chromatographic separations using ethoxynonafluorobutane as hexane alternative. II. Liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry applications with methanol gradients. J Chromatogr A 1033 321. 

Krahn M, Ylitalo G, Buzitis J, et al. 1993. Rapid high-performance liquid-chromatographic methods that screen for aromatic-compounds in environmental-samples. Journal of Chromatography 642(1-2) 15-32. 

D. Anderson, D. R. Ferry, R. J. Knox et al. High-performance liquid chromatographic method for sensitive determination of the alkylating agent CB1954 in human plasma. Journal of Chromatography. B, Biomedical Sciences and Applications, 1999, 731(2), 293-298. 

Snyder, L.R. and Dolan, J.W. (1996) Initial experiments in high-performance liquid chromatographic method development. 1. Use of a starting gradient run. Journal of Chromatography. A, 721, 3-14. 

Simpson, R. C. and Brown, R. R., Development of a microbore high-performance liquid chromatographic system for biological applications. Journal of Chromatography 385,41-54, 1987. 

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