Chicken embryo-related cells

Conti C, Superti F, Tsiang H. Membrane carbohydrate requirement for rabies virus binding to chicken embryo related cells. Intervirology. 1986 26(3) 164-168. 

Cardoso TC, da Silva LH, da Silva SE, et al. Chicken embryo related (CER) cell line for quantification of rabies neutralizing antibody by fluorescent focus inhibition test. Biologicals. 2006 34(l) 29-32. 

Lukert (1972) made the interesting observation, which may not, however, be directly related to substrate specificity, that the attachment of avian, infectious bronchitis virus to monolayers of chicken embryo kidney cells was inhibited not only by the addition of free or bound sialic acid, but by sulfhydryl-containing compounds. Receptors could be destroyed by neuraminidase or p-hydroxymercuribenzoate treatment. The adsorption of Newcastle disease virus, however, was not affected by the addition of sulfhydryl compounds. 

Another interesting question relates to the problem of cellular differentiation. The fact that undifferentiated cells contain their contractile protein in a nonstructured form might lead to the speculation that this represents the state of a precursor of the structured actomyosins in the course of cellular development. Actually, this does not seem to be true. Holtzer et al. (1957) working with fluorescent antimyosin antibodies were able to show that, whenever myosin appears in the cellular protoplasm of the chicken embryo myoblasts, it does so already incorporated into a fibrillar structure. 

---