Antimyosin antibody

Another interesting question relates to the problem of cellular differentiation. The fact that undifferentiated cells contain their contractile protein in a nonstructured form might lead to the speculation that this represents the state of a precursor of the structured actomyosins in the course of cellular development. Actually, this does not seem to be true. Holtzer et al. (1957) working with fluorescent antimyosin antibodies were able to show that, whenever myosin appears in the cellular protoplasm of the chicken embryo myoblasts, it does so already incorporated into a fibrillar structure. 

The application of antibodies in cardiovascular targeting in vivo originated with the experimental demonstration of the feasibility of using radiolabeled antimyosin antibody for diagnosis of acute myocardial infarction in 1976. Since then, the use of antibodies in the cardiovascular system has encompassed imaging of myocarditis,heart transplant rejection, dilated cardiomyopathy, alcohol induced cardiomyopathy,adriamycin cardiotoxicity, various other cardiomyopathies, vascular clots, atherosclerotic lesions,and even certain cancers such as soft tissue sarcomas.f Yet the best characterized and studied antibody for cardiovascular diagnostic targeting is monoclonal antimyosin Fab for its exquisite specificity... 

Targeted Delivery of Radiolabeled Antimyosin Antibody in Acute Myocardiai Infarction... 

Ballester, M. Marti, V. Carrio, I. Spectrum of alcohol-induced myocardial damage detected by indium-111-labeled monoclonal antimyosin antibodies. J. Am. Coll. Cardiol. 1997, 29 (1), 160-167. 

Narnia, J. Torchihn, V.P. Petrov, A. Khaw, S. Trubetskoy, V.S. O Dormell, S.M. Nossiff, N.D. Khaw, B.A. In-vivo targeting of acute myocardial infarction with negative charge, polymer-modified antimyosin antibody use of different cross-linkers. J. Nucl. Cardiol. 1995, 2, 26-34. 

BaUester, M. Obrador, D. Carrio, I. Caralps-Riera, J.M. Indium-111 monoclonal antimyosin antibody studies in the diagnosis of rejection and management of patients after heart transplantation. In Monoclonal Antibodies in Cardiovascular Disease Khaw, B.A., Narula, J., Strauss, H.W., Eds. Lee Febiger Philadelphia, 1994 79-98. 

J. M. Early postoperative reduction of monoclonal antimyosin antibody uptake is associated with absent rejection related compUcations after heart transplantation. Circulation 1992, 85, 61-68. 

Casans, I. Villar, A. Almenar, V. Blanes, A. Lyme myocarditis diagnosis by indium-111 antimyosin antibody scintigraphy. Eur. J. Nucl. Med. 1989, 15, 330-331. 

Myosin-specific monoclonal 2G42D7, an immunoglobulin G (IgG)-l murine antibody has an apparent affinity of approximately 1 xlO L/mol [30, 31]. Antimyosin antibody 2G42D7 is produced in bioreactors (Cell-max QUAD, Cellco Inc. Laguna Hills, California) with Dulbecco s Modified Eagle s Medium (DMEM) (Sigma-Aldrich, MO,USA) supplemented with 10%... 

Antimyosin antibody is modified with N-glutarylphosphatidyl ethanolamine (NGPE Avanti Polar Lipids, Hercules, California) (22).Briefly ... 

Torchilin VP, Khaw BA, Smirnov VN, Haber E (1979) Preservation of antimyosin antibody activity after covalent coupling to liposomes. Biochem Biophys Res Commun 89 1114-1119... 

To target liposomes to the infarcted myocardium, attempts have also been made to utilize AM antibody with liposomes (40). Antimyosin antibody (see... 

The hallmark of necrotic cell death is the loss of cell membrane integrity as evidenced by the presence of cell membrane lesions. Antimyosin antibody, a... 

Khaw, B. A. (1994) Antimyosin antibody for the diagnosis of acute myocardial infarction experimental validation, in Monoclonal Antibodies in Cardiovascular Diseases (Khaw, B. A., Narula, J., and Strauss, H. W., eds.), Lea and Febiger, Malvern, PA, pp. 15-29. 

Syhre R, Seifert S, Spies H, et al (1998) Stability versus reactivity of 3+1 mixed-ligand techne-tium-99m complexes in vitro and in vivo. Eur J Nucl Med 25 793-796 Taillefer R, Boucher L, Lambert R et al (1995) Technetium-99m antimyosin antibody (3-48) myocardial imaging human biodistribution, safety and clinical results in detection of acute myocardial infarction. Eur J Nucl Med 22 453-464... 

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