Cereus stearothermophilus

The basic microbial inhibition assay format involves a standard culture of a test organism, usually Bacillus stearothermophilus, Bacillus subtilis, Bacillus cereus, Micrococcus luteus, Escherichia coli, Bacillus megatherium, or Strepto-793... 

In the six-plate test, the sample is brought in a punch hole or a paper disc on each of the six test plates that have been inoculated with Bacillus cereus. Bacillus subtilis (pH 6), Bacillus subtilis (pH 8), Sarcina lutea, Escherichia coli, and Bacillus stearothermophilus, respectively (33). Once the samples have been introduced, the plates are incubated for the required time at the required temperature. If, on one of the plates, a zone of inhibition of 1.0 mm is observed around the disks or holes, the result of the test is considered positive, provided that interferences have been excluded. 

LeuDH from B. stearothermophilus had already been cloned and overexpressed [20, 331 during early studies. Recently, the production of recombinant enzyme from B. cereus even on a large scale has been demonstrated 34, 35. ... 

The substrate specificity of LeuDHs, catalyzing mainly branched-chain a-keto acids to the a-amino acids, has been investigated by Zink and Sanwal (1962) 36) and subsequently by Schiitte et al. (1985 B. cereus) t6, Ohshima and Soda (1989 Bacillus stearothermophilus and Bacillus sphaericus)1S1, Nagata et al. (1990 Bacillus DSM 7330) l37 Misono et al. (1990 Corynebacterium pseudodiphtheriticum) 38 and by Bommarius et al. (1994 Bacillus stearothermophilus)l39. In addition to the proteino-genic amino acids valine, leucine, and isoleucine, unnatural amino acids such as tert-leucine1401 or L-(3-hydroxy-valine[411 can be synthesized. 

Wescott, G.G. Fairchild, T.M. Foegeding, P.M. Bacillus cereus and Bacillus stearothermophilus spore activation in batch and continuous flow systems. J. Food Sci. 1995, 60, 446-450. 

Micobacterium tuberculosis Helicobacter pylor Micobacterium tuberculosis Micobacterium tuberculosis Micobacterium tuberculosis Mycobacterium smegmatis M. tuberculosis Streptococcus faecalis Staphylococcus aureus Bacillus cereus B. stearothermophilus B. subtilis Citrobacter freundii Enterobacter cloacae Escherichia coli Klebsiella pneumoniae Morganella morganii Proteus mirabilis Pseudomonas aeruginosa Shigella dysenteriae Salmonella typhi MRSA... 

Escherichia coli Salmonella typhimurium Staphylococcus epidermis Staphylococcus aureus Staphylococcus epidermis Staphylococcus aureus Bacillus cereus B. megaterium B. stearothermophilus B. subtilis... 

Kreft J, Berger H, Haertlein M, Mueller B, Goebel G, Weidinger W (1983) Qoning and expression in E. coli and Bacillus subtilis of the hemolysin determinant from Bacillus cereus. J Bacteriol 155 681-689 Lapidot A, Mechaly A, Shoham Y (1996) Overexpression and single-step purification of a thermostable xylanase from Bacillus stearothermophilus T-6. J Biotechnol 51 259-264... 

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