Cellulosic matrices

The construction of hybrid proteins containing bacterial CBDs may provide a cheap generic method for enzyme immobilization and/or purification using cellulosic matrices. The CBD can be fused at the amino or carboxyl terminus, as in the parent cellulase, to suit individual applications. We have constructed model fusion proteins using the C. fimi CBDs to demonstrate this potential. 

Feely, L. C., and Davis, S. S. Correlation of phenylpropanolamine bioavailability with gastrointestinal transit by scintigraphic monitoring of mIn-labeled hydroxypropyl-cellulose matrices. Pharm. Res. 6 274-278, 1989. 

Pham, A. and Lee, P., Probing the mechanisms of drug release from hy-droxypropylmethyl cellulose matrices, Pharmaceutical Research, Vol. 11, No. 10, 1994, pp. 1379-1384. 

Lippold, B.C. Mockel, J.E. Pulsatile release from laminated methylhydroxypropyl cellulose matrices with KCl as model drug. Acta Pharm. Technol. 1990, 36 (2), 97-98. 

Resins with cellulosic matrices are much more hydrophilic and these do not tend to denature proteins. Cellulosic resins have been used extensively in the laboratory analyses of biological materials, enzyme immobilizations and small scale preparations. The low capacity and poor flow characteristics have limited the usefulness of these matrices for larger applications. 

Recently, diethylaminoethyl (DEAE) silica gel was shown to be an improvement over typical cellulosic-matrices resins for the separation of acidic and neutral lipids from complex ganglioside mixtures. The specific advantages claimed were ... 

Nilesh A., Kamat, M., Arvind, L. (2004). Expanded bed affinity purification of bacterial a-amylase and Cellulase on composite substrate analogue-Cellulose matrices. Process Biochemistry, 39, 565-570. 

Several intermediates are available commercially for instance, CNBr-Sepharose, Sepharose and Bio-Gel P (polyamide) intermediates with reactive spacer groups attached, and Sepharose already coupled to a ligand specific for polysaccharides and glycoproteins. An outline of the reactions developed to couple ligands to Sepharose is illustrated in Figure 4.15 dextran and cellulose matrices can be derivatised in a similar manner. 

Solinis, M.A. Lugara, S. Calvo, B. Hernandez, R.M. Gascon, A.R. Pedraz, J.L. Release of salbutamol sulfate enantiomers from hydrox5 propylmethyl-cellulose matrices. Int. J. Pharm. 1998, 161 (1), 37-43. 

Katzhendler, I. Mader, K. Friedman, M. Structure and hydration properties of hydroxypropyl methyl-cellulose matrices containing naproxen and naproxen sodium. Int. J. Pharm. 2000, 200 (2), 161-179. 

Chang CY, Peng J, Zhang LN, Pang DW (2009) Strongly fluorescent hydrogels with quantum dots embedded in cellulose matrices. J Mater Chem 19 7771—7776... 

Sodium borohydride has been incorporated into alumina (Hodosan and Servan, 1969), cellulose matrices (Perrier and Benerito, 1976), and ion-exchange resins (Gibson and Bailey, 1977). The latter reagent was readily prepared using Amberlite IRA-400 and Amberlyst A-26. A more extensive survey of the properties of this material has been published (Cook et al., 1978 Cook, 1978). The borohydride resin (Resin-Borohydride) is a developmental product of Ventron Corporation, Beverly, Massachusetts. 

Cellulose is the most abundant biopolymer on earth. It can be used in different applications, namely in the form of fibers, and cellulose can be converted into numerous cellulose derivatives. Cellulose micro- and nanofibers have been the subject of intense research in the field of composites. Cellulose derivatives can show liquid crystalline chiral nematic phases, which can be used for the production of diverse composite systems. All-cellulosic composites based on liquid crystalline cellulosic matrices reinforced by cellulose micro- and nanofibers can show enhanced mechanical properties due to fiber orientation induced by the liquid crystalline matrix. Cellulose-based fibers electrospun from liquid crystalline phases can develop different structures, which are able to mimic the shape of plant tendrils on the nano- and microscale, opening new horizons for ceDulosic membrane applications. 

Costa 1, Almeida PL, Filip D, Figueirinhas JL, Godinho MH (2006) Tunable topographical cellulose matrices for electro-optical liquid crystal cells. Opto-Electron Rev 14(4) 299-303 Costa I, Filip D, Figueirinhas JL, Godinho MH (2007) New cellulose derivatives composites for electro-optical sensors. Carbohydr Polym 68(1) 159-165 Credou J, Berthelot T (2014) Cellulose from biocompatible to bioactive material. J Mater Chem B 2(30) 4767-4788... 

This oxidation is monitored with a pC>2 electrode whose response diminishes widi ethanol concentration. Cellulose matrices treated with... 

---