Cellulose for ion exchange

Examples of successful fractionations of aquatic humic substances where only one fractionation mechanism was operative include utilization of the hydrophobic properties of XAD resins (Mantoura and Riley, 1975 Aiken et al., 1979), hydrogen bonding of weak-acid functionalities of humic constituents to weak-base anion-exchange resins (Kim et al., 1976), and use of ion-exchange celluloses for ion-exchange fractionation of aquatic humic substances without hydrophobic matrix adsorption (Sirotkina et al., 1974). These examples of successful fractionations demonstrate the potential for chromatography of aquatic humic substances when fractionations are designed carefully to avoid undesirable interactions. 

TABLE 13. MODIFIED FIBROUS CELLULOSES FOR ION-EXCHANGE CHROMATOGRAPHY ... 

Alumina, which is discussed in greater detail in Chapter 5, and cellulose are the two most often used nonsilica-based sorbents. Alumina has been used to separate compounds such as fat-soluble vitamins, alkaloids, and antibiotics. Cellulose-based ion-exchange layers have wide application for sulfonamides, nucleic acids, and steroid sulfates. 

Cellulose-based monoliths prepared from cross-linked sponge-like regenerated cellulose with a continuous, interconnected, open pore stmcture (50-300 p.m) are commercialized by Sepragen under the trade name Seprasorb and are available for ion-exchange chromatography. 

Cellulose ion exchangers (e.g., diethylaminoethyl cellulose) and ion-exchange resins have been widely used as stationary phases for TLC separation of untreated amino acids. 

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