Diethylaminoethyl -cellulose

DEAE-celluloae See diethylaminoethyl cellulose. de,e a,e sel-y3,los dealkalization chem 1. Removal ofalkali. 2. Reduction ofalkalinity, as in the process of neutralization. de,al-k3T3 za-sh3n dealkylate chem To remove alkyl groups from a compound. de al-k3,lat dealuminization chem Removal of aluminum., de-3,lu-m3-n3 za-sh3n deamidation org chem Removal of the amido group from a molecule. de,am-o da-shon ... 

Wei, T.-M. 1982. Batch fractionation of casein with diethylaminoethyl cellulose. M. S. thesis, University of Illinois, Urbana. 

Murine epidermal growth factor is a polypeptide containing 53 residues. Its molar mass is 6040 g/mol. Chromatography on diethylaminoethyl cellulose produced a single peak. The isocratic RPC of this seemingly homogeneous material on a C 18 column (300 x 7.8 mm) yielded two clearly separated peaks (Fig. 19)77). The eluent was water — acetonitrile (74 26), 0.04 M in triethylamine acetate, pH = 5.6. The structure of the p component is identical with that of the a component but does not possess the Asn residue at chain position 1. 

Fig. 19. Efficiency of RPC in protein separation. Isocratic RPC separation of murine epidermal growth factor (after elution as a single peak from a diethylaminoethyl cellulose column). Column RP 18 (300x7.8 mm dP = 10 pm) 40 °C eluent 0.04 M triethylamine acetate in water — acetonitrile (74 26), pH 5.6 flow rate 0.8 ml/min UV detection at 254 nm, 0.05 AUFS injection 100 pg in 100 pi. The a peak was due to the polypeptide of 53 amino acid residues (M =6040 g/mol), the p component did not contain the asparagine residue in position 1. (From Ref.77) with permission)...

Amarita et al. [48] Lactose Milk Lactases (Lactozym and / -galactosidase of Enterobacter agglomerans) adsorbed onto diethylaminoethyl cellulose (DE-52), and confined between the surface of a gas-permeable membrane of a C02 electrode and a dialysis membrane C02 potentiometric electrode ... 

A solution containing egg albumin (pH/ = 4.6), /3-lactoglobulin (pH/ = 5.2), and chymotrypsinogen (pH/ = 9.5) was loaded onto a column of diethylaminoethyl cellulose (DEAE-cellulose) at pH 5.4. The column was then eluted with pH 5.4 buffer, with an increasing salt concentration. Predict the elution pattern. 

Class I Not more than 50% of the color is bound by DEAE (diethylaminoethyl) cellulose, and not more than 50% of the color is bound by phosphoryl cellulose. 

Sephadex and 0-(2-diethylaminoethyl)cellulose with a-bromotolyl-boronic acid, and studied the separation of free sugars92,93 and nucleosides and nucleotides93,94 on them at various pH values and with different eluants. Compounds containing cis-1,2-diol groupings were, again, the most strongly bound. 

Chromatography of polysaccharides on silica gel and on carbon columns has been used to a limited extent. The use of ion-exchange resins has been investigated by Deuel and coworkers. The colunms had low capacities, and part of the material appeared to be irreversibly adsorbed. Deuel and coworkers, however, have reported better results when columns of cationic derivatives of cellulose, for instance (diethylaminoethyl)-cellulose, are used. Electrophoresis both by the Tiselius method on columns and on filter paper or glass-fiber sheets can give good separa-... 

NMR, nuclear magnetic resonance mV, millivolt pCMB, p hloromercuribenzoate pCMS, p-chloromercuriphenyl sulfonate PMS, phenazine methosulfate EDTA, ethylenediaminetetraacetate SDS, sodium dodecyl sulfate TTFA, 2-thenoyltriflu-oroacetone ETP, submitochondrial (electron transfer) particles MVH, reduced methyl viologen DEAE-cellulose, diethylaminoethyl cellulose succ, succinate APS, adenosine S -phosphosulfate PAPS, 3 -phosphoadenosine 5 -phosphosulfate. Other abbreviations are standard [see JBC 244, 2 (1969)]. 

Diethyl azodicarboxylate Diethylaminoethyl cellulose Diethylaluminium hydride Diethylaluminium iodide... 

Cellulose ion exchangers (e.g., diethylaminoethyl cellulose) and ion-exchange resins have been widely used as stationary phases for TLC separation of untreated amino acids. 

DEAE-cellulose. (diethylaminoethyl cellulose). A cellulose ether containing the group (C2H5)2NCH2CH2 bound to the cellulose in an ether linkage. An anionic ion-exchange material. 

Industrial applications include anion-exchangers diethylaminoethyl cellulose (DEAE-cellulose) and the cation-exchanger carboxymethyl cellulose (CM-cellulose)... 

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