Cell staining cytospin

Determine differential cell counts on cytospin smears of lung washes (3 min at 100xjj) stained by the Wright-Giemsa method. 

Differential cell counts of the BAL and peritoneal fluids are performed on cytospin-prepared slides. Differential counts of leukocytes in peripheral blood are performed on blood smears. The slides are stained with Diff-Quik (American Scientific Products, McGaw Park, IL) and at least 200 cells are counted per sample to determine the percent of neutrophils, macrophages, lymphocytes, and other cells. 

Zymed antibody sensitivity was 100% compared with a Chemicon sensitivity of 74%. Focal, weak staining of adenocarcinoma was seen in 4% and 9%, respectively. Nagel and colleagues had a similar experience with the study of cytospins. They found that the sensitivity for mesothelial cells was 93%, with 5% of tumor cells immunostaining." In a recent study by Yaziji and associates, calretinin was one of three antibodies (along with BG8 and MOC31) identified in the evaluation of a... 

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