Cells cytology

The aim of this study was to examine and report changes in the oral cavity epithelial cell cytology (histopathological changes) during 24-month chronic administration of Oralin spray formulation, compared to a placebo formulation. The secondary objective was to examine hematological changes and any hepatotoxicity. 

Smolov, A. P. and Opanasenko, V. K. Ammonium factor in the vital activity of plant cell. Cytology, 51, 358-366 (2009). 

Filtering cells and cell fractions from fluid media. These particles, after concentration by filtration, may be examined through subsequent quantitative or qualitative analysis. The filtration techniques also have applications in fields related to immunology and implantation of tissues as well as in cytological evaluation of cerebrospinal, fluid. 

Protoanemonin, which has been isolated from Anemone pulsatilla and Ranunculus spp., was reported to inhibit root growth by slowing down metabolism and blocking mitosis 35). Erickson and Rosen 35) observed cytological effects in corn root tips at concentrations of 10M and lower. Cells undergoing division appeared to accumulate in the interphase or prophase stages. Metaphase, anaphase, and telophase stages were not observed. Cytoplasmic and vacuolar structures were disturbed and the presence of mitochondria could not be demonstrated in treated tissue. Thimann and Bonner 141) reported that protoanemonin was 10 to 30 times more inhibitory than coumarin in coleoptile and split pea stem tests, and that BAL prevented the inhibitory action. 

Uncultured cells have also been used to a limited extent for fetal sex determination. This Is possible because the cells can be appropriately stained and examined for the presence of sex chromatin, characteristic of female cells, and for the fluorescent Y-body, characteristic of male cells (33,34). However, these cytologic techniques are not completely reliable, and It Is still desirable to determine fetal sex by direct chromosome analysis (35). 

Gerble, A. B. Melancon, S. B. Ryan, C. and Nadler, H. L. "Cultivated Eplthellal-Llke Cells and Fibroblasts from Amniotic Fluid Their Relationship to Enzymatic and Cytologic Analysis". Am. J. Obstet. Gynecol., (1972), 114, 314-320. 

Detects cancerous cells that become disloged from the airways into the sputum. Sputum cytology is useful because it is not invasive, although it has much lower sensitivity for detecting cancer. 

Bird, A.F. (1961) The ultrastracture and histochemistry of a nematode-induced giant cell. Journal of Biophysical and Biochemical Cytology 11, 701—715. 

Gordon, R. and Drum, R.W. (1994) The chemical basis of diatom morphogenesis. International Review of Cytology-A Survey of Cell Biology, 150, 243-372. 

Recently, the use of AR has extended into several other areas, yielding interesting information for cytology, fresh cell/tissue sections, and fluorescence IHC (fluorescence in situ hybridization [FISH]), in addition to adaptations of the method for extraction of nucleic acids and proteins from FFPE tissues for use with modern methods of molecular analysis. In this chapter, the emphasis is on expanded applications in diagnostic cytology, fresh frozen cell/... 

Boon ME, Beck S, Kok LP. Semiautomatic PAPNET analysis of proliferating (MiB-l-positive) cells in cervical cytology and histology. Diagn. Cytopathol. 1995 13 423 128. 

General cytoplasmic stains are much more problematic in that a single section never contains an entire cell, even for very small cells. The only strategy that can be used for these stains is to determine a concentration per area, given that there will be variation due to the variation in section thickness and perhaps also to cytoplasmic localization of stained product. And there is simply no accurate way to determine the total amount of material within a single cell cytoplasm, unless one has a cytological (intact) specimen. 

However, ICC is not used as frequently in cytology as IHC in surgical pathology due to some technical issues, such as scanty or lack of diagnostic material in cell blocks or cytospins, and questionable reliability of immunos-taining on smears. 

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