Fluorescent-activated cell sorting

The ability to display proteins on the surface of an organism has been exploited as a screening method, typically in conjunction with fluorescence-activated cell sorting (FACS) [37,47,48]... 

Becker, S., Schmoldt, H.U., Adams, T.M. et al. (2004) Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts. Current Opinion in Biotechnology, 15, 323-329. 

The chemical composition of particles can be just as varied as their shape. Commercial particles can consist of polymers or copolymers, inorganic constructs, metals and semiconductors, superparamagnetic composites, biodegradable constructs, and synthetic dendrimers and dendrons. Often, both the composition of a particle and its shape govern its suitability for a particular purpose. For instance, composite particles containing superparamagnetic iron oxide typically are used for small-scale affinity separations, especially for cell separations followed by flow cytometry analysis or fluorescence-activated cell sorting (FACS). Core-shell semiconductor particles, by... 

Abou-Samra, A.B., Freeman, M., Juppner, H., Uneno, S., and Segre, G.V. (1990) Characterization of fully active biotinylated parathyroid hormone analogs. Applications to fluorescence activated cell sorting of parathyroid hormone receptor bearing cells./. Biol. Chem. 265, 58-62. 

J. Kruger, K. Singh, A. O Neill, C. Jackson, A. Morrison, and P. O Brien, Development of a microfluidic device for fluorescence activated cell sorting. J. Micromech. Microeng. 12, 486-494 (2002). 

Wang, J. L., and McDaniel, M. L. (1990). Secretagogue-induced oscillations of cytoplas mic Ca2+ in single fi and a cells obtained from pancreatic islets by fluorescence-activated cell sorting. BitKhem. Biophys. Res. Commun. 166, 813-818. 

Immunoassay kit, where the antibodies of 8-oxoguanine (96) are conjugated with fluorescein isothiocyante (97) as fluorophore and combine with the oxidized DNA. Detection of the greenish fluorescence is by fluorescence microscopy for tissues or by fluorescence-activated cell sorting for cell suspensions236. 

The procedure is cheaper than fluorescence-activated cell-sorting (FACS)... 

As indicated above, one major advantage of bacterial and other cell-based surface display formats lies in the ability to use fluorescence-activated cell sorting for high-throughput library screening. With modem FACS equipment, such as the Cytomation MoFlo or the FACS Vantage from Beckton-Dickinson, sorting rates of up to 100 000 events per second are possible [10]. 

Asami, M., Nakatsuka, T., Flayashi, T., Kou, K., Kagawa, H. and Agata, K. (2002) Cultivation and characterization of planarian neuronal cells isolated by fluorescence-activated cell sorting (FACS). Zoological Science 1 9, 1 257-1265. 

Herzenberg LA, Sweet RG, Herzenberg LA (1976). Fluorescence-activated cell sorting. Sci. Am. 234 108-115. 

Malatesta, P., Hartfuss, E., and Gotz, M., Isolation of radial glial cells by fluorescent-activated cell sorting reveals a neuronal lineage, Development, 127, 5253, 2000. 

Pharmacokinetic data analysis requires determination of the analyte in various body fluids. In the case of therapeutic antibodies, serum is the most common matrix to be analyzed. For a critical interpretation of pharmacokinetic data the chosen bioanalytical methods must be considered. The most frequently used for mAbs include enzyme-linked immunosorbent assay (ELISA), capillary electrophoresis (CE)/polyacrylamide gel electrophoresis (PAGE), fluorescence-activated cell sorting (FACS), and surface plasmon resonance (SPR). The challenges and limitations of bioanalytical methods used for the analysis of mAb concentrations are discussed in detail in Chapter 6. 

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