Cell wall, protein sorting

K. Miscellaneous Issues 1. Sorting of Yeast Cell Wall Proteins... 

As described in Section II,A, gram-positive bacteria have only one membrane (the cytoplasmic membrane). Therefore, the translocation through the Sec pathway directly leads proteins to be secreted (Simonen and Palva, 1993 Nagarajan, 1993). The issue of protein sorting into the cell wall is described in a separate section. 

In spite of the variety of appearances of eukaryotic cells, their intracellular structures are essentially the same. Because of their extensive internal membrane structure, however, the problem of precise protein sorting for eukaryotic cells becomes much more difficult than that for bacteria. Figure 4 schematically illustrates this situation. There are various membrane-bound compartments within the cell. Such compartments are called organelles. Besides the plasma membrane, a typical animal cell has the nucleus, the mitochondrion (which has two membranes see Fig. 6), the peroxisome, the ER, the Golgi apparatus, the lysosome, and the endosome, among others. As for the Golgi apparatus, there are more precise distinctions between the cis, medial, and trans cisternae, and the TGN trans Golgi network) (see Fig. 8). In typical plant cells, the chloroplast (which has three membranes see Fig. 7) and the cell wall are added, and the lysosome is replaced with the vacuole. 

Schneewind, O., Mihaylova-Petkov, D., and Model, P. (1993). Cell wall sorting signals in surface proteins of gram-positive bacteria. EMBO J. 12, 4803-4811. 

Schneewind, O., Model, P., andFischetti, V. (1992). Sorting of protein a to the staphylococcal cell wall. Cell 70, 267-281. 

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