Fluorescent cell-sorting

Measurements of particular properties of a compound or substance (enzyme kinetics, reaction kinetics, FACS, fluorescence-activat cell sorting, assay). 

The ability to display proteins on the surface of an organism has been exploited as a screening method, typically in conjunction with fluorescence-activated cell sorting (FACS) [37,47,48]... 

Becker, S., Schmoldt, H.U., Adams, T.M. et al. (2004) Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts. Current Opinion in Biotechnology, 15, 323-329. 

The labeled glycans may be analyzed by cell sorting after staining with fluorescently modified streptavidin. Alternatively, the cells may be lysed and the labeled glycans isolated using immobilized streptavidin. 

The chemical composition of particles can be just as varied as their shape. Commercial particles can consist of polymers or copolymers, inorganic constructs, metals and semiconductors, superparamagnetic composites, biodegradable constructs, and synthetic dendrimers and dendrons. Often, both the composition of a particle and its shape govern its suitability for a particular purpose. For instance, composite particles containing superparamagnetic iron oxide typically are used for small-scale affinity separations, especially for cell separations followed by flow cytometry analysis or fluorescence-activated cell sorting (FACS). Core-shell semiconductor particles, by... 

Abou-Samra, A.B., Freeman, M., Juppner, H., Uneno, S., and Segre, G.V. (1990) Characterization of fully active biotinylated parathyroid hormone analogs. Applications to fluorescence activated cell sorting of parathyroid hormone receptor bearing cells./. Biol. Chem. 265, 58-62. 

J. Kruger, K. Singh, A. O Neill, C. Jackson, A. Morrison, and P. O Brien, Development of a microfluidic device for fluorescence activated cell sorting. J. Micromech. Microeng. 12, 486-494 (2002). 

Wang, J. L., and McDaniel, M. L. (1990). Secretagogue-induced oscillations of cytoplas mic Ca2+ in single fi and a cells obtained from pancreatic islets by fluorescence-activated cell sorting. BitKhem. Biophys. Res. Commun. 166, 813-818. 

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