Ultra high throughput screening

Becker, S., Schmoldt, H.U., Adams, T.M. et al. (2004) Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts. Current Opinion in Biotechnology, 15, 323-329. 

Mere, L., Bennett, T., Coassin, P., Hamman, B., Rink, T., Zimmerman, S. and Nelulescu, P. (1999). Miniturized FRET assays and microfluidics Key components for ultra-high-throughput screening. Drug Discov. Today 4, 363-9. 

Sundberg, S.A. High-throughput and ultra-high-throughput screening solution- and cell-based approaches. Curr. Opin. Biotechnol. 2000, 11, 47-53. 

Fig. 28 Schematic view of the Zeiss Plate Vision instrument which is state-of-the art for ultra-high throughput screening (uHTS) for drug discovery. The instrument resembles a 96-well parallel microscope the light of a excitation source (Xe-lamp or pulsed laser) is expanded to illuminate a microtiter plate. The excitation is structured into 96 channels by a mini-lens array (MLA) and focused into the well with a detection volume of < 100 nL. All 96 channels are read simultaneously by a gated, intensifed CCD. With this fast detector and the pulsed laser excitation, the instrument can be used to carry out miniaturized, 96 parallel lifetime measurements in microtiter plate format with nanosecond time resolution or time-gated detection [190]...

Sundberg, S. A. High-Throughput and Ultra-High-Throughput Screening Solution- and Cell-Based Approaches. 

Marron, B. E., Jayawickreme, C. K., Going to the well no more lawn format assays for ultra-high-throughput screening, Curr. Opin. Chem. Biol. 2003, 7, 395—101. 

Winkler, T., Kettling, U., Koltermann, A., and Eigen, M. (1999). Confocal fluorescence coincidence analysis an approach to ultra high-throughput screening. Proc. Natl. Acad. Sci. USA 96, 1375-1378. 

Fig. 8.5. Miniaturized biodevices for ultra-high-throughput screening. (A) Piezo-electric ink-jet pipettor that allows pipetting down to the pico-liter scale with speeds up to 10,000 drops per second and an accuracy of 3 % [97] (B) Sample carrier containing six segments of a silicon wafer. Each segment comprises 900 reaction compartments with a maximum volume of 120 nl each [28].

Mere L, Bennett T, Coassin P, England P, Hamman B, Rink T, Zimmerman S, Negulescu P. Miniaturized FRET assays and microfluidics key components for ultra-high-throughput screening. 94. Drug Discov. Today 1999 4 363-369. 

U Haupts, M Rudiger, AJ Pope. Macroscopic versus microscopic fluorescence techniques in (ultra)-high-throughput screening. Drug Discov Today 1 3—9, 2000. 

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