Cell-map proteomics

Cell map proteomics reveals the static proteome of a whole organism, tissue, cell or organelle, while expression proteomics investigates changes in a proteome to cellular cues (Godovac-Zimmermann and Brown, 2001). Functional and structural proteomics refers to the investigation of individual proteins such as interactions with ligands. 

Among several different descriptions of the nature and purpose of proteomics, one of the most useful is the separation into expression proteomics and cell-map proteomics (Blackstock. 1999) ... 

While the concept of a protein catalog undoubtedly existed, at least in the minds of some people, prior to the coining of the term proteome, the realization that protein-protein interactions were a dominant feature of proteome function was certainly considerably less clear. However, such is the case and the second part of the definition requires a detailed knowledge of all of these recognitive events, both stable and transient. The elucidation of these interactions and the underlying networks that they describe have been termed cell-mapping proteomics (Blackstock Weir, 1999). This in fact has been a quite productive area of proteomic research, as described below. 

Lee CL, Hsiao HH, Lin CW, Wu SP, Huang SY, Wu CY, Wang AH, Khoo KH. Strategic shotgun proteomics approach for efficient construction of an expression map of targeted protein families in hepatoma cell lines. Proteomics 2003 3(12) 2472-86. 

Au, C.E., Bell, A.W., Gilchrist, A. et al. 2007. Organellar proteomics to create the cell map. Curr Opin Cell Biol 19 376-385. 

D) polyacrylamide gels. These types of experiments have been performed for more than twenty years to build databases of proteins expressed from certain cell or tissue types (Anderson and Anderson, 1996 O Farrell, 1975). Although this remains an important component of proteomics research, the field has expanded due to the development of additional technologies. Proteomics can be broadly divided into two areas of research (i) protein expression mapping, and (ii) protein interaction mapping. 

Proteomic Mapping and Clustering of Multiple Samples—Application to Ovarian Cancer Cell Lines... 

Qi SY et al. Proteome of Salmonella typhi-murium SL1344 identification of novel abundant cell envelope proteins and assignment to a two-dimensional reference map. J Bacterid 1996 178 5032-5038. 

Lewis TS et al. Identification of novel MAP kinase pathway signaling targets by functional proteomics and mass spectrometry. Mol Cell 2000 6 1343-1354. 

Proteomics can make a key contribution in the identification of interacting protein partners and create a protein-protein interaction map of the cell (Lamond and Mann, 1997 Neubaer et ah, 1997 Blackstock and Weir, 1999 Link et ah, 1999). This would be of immense value to understanding the biology of the cell and can be further exploited for drug development. The simplest way to study such interaction is to purify the entire multiprotein complex by affinity-based methods. The members of the multiprotein complex can then be identified by mass spectrometry. 

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