Proteomics, shotgun approach

Storms HF, van der HR, Tjaden UR, van der GJ. Capillary isoelectric focusing-mass spectrometry for shotgun approach in proteomics. Electrophoresis 2004 25 3461-7. 

Castro-Perez JM, Kamphorst J, De Groot J, Lafeber F, Goshawk J, Yu K, et al. Comprehensive LC—MS lipidomic analysis using a shotgun approach and its application to biomarker detection and identification in osteoarthritis patients. J Proteome Res 2010 9 2377-89. 

In the gel-free approaches, also referred as shotgun proteomics, proteins in a mixture are digested and the peptides released separated by reverse-phase HPLC, either alone or combined with strong cation exchange chromatography... 

The study of proteins is especially interesting in food safety because they may act as toxins, antinutrients, or allergens (53). Proteomics, a high-throughput technology able to quantify hundreds of proteins simultaneously, has become very important in comparative studies of GM plants and their nonmodified counterparts (54). Two conceptually different strategies can be followed in comparative proteomics the shotgun and the bottom-up approaches. 

The second approach, a shotgun proteomic analysis (LC-MS/MS) of tryptic solution-digests of isolated synaptosomes, identified 209 unique proteins. When the two sets of identified proteins are compared, 46 were found to be common. Accounting for this intersection, the total number of unique proteins identified by the... 

Sun B, et al. Shotgun glycopeptide capture approach coupled with mass spectrometry for comprehensive glycoproteomics. Mol. Cell. Proteomics 2007 6 141-9. 

The focus in this text is on the application of LC-MS technology. Three general approaches to proteome mining can be discriminated, which are related to the three general approaches to protein identification bottom-up, top-down, and shotgun. The latter essentially is a bottom-up approach, but is discussed separately. 

Lee CL, Hsiao HH, Lin CW, Wu SP, Huang SY, Wu CY, Wang AH, Khoo KH. Strategic shotgun proteomics approach for efficient construction of an expression map of targeted protein families in hepatoma cell lines. Proteomics 2003 3(12) 2472-86. 

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