Cell labeling magnetic

Most microscopic theories of adsorption and desorption are based on the lattice gas model. One assumes that the surface of a sohd can be divided into two-dimensional cells, labelled i, for which one introduces microscopic variables Hi = 1 or 0, depending on whether cell i is occupied by an adsorbed gas particle or not. (The connection with magnetic systems is made by a transformation to spin variables cr, = 2n, — 1.) In its simplest form a lattice gas model is restricted to the submonolayer regime and to gas-solid systems in which the surface structure and the adsorption sites do not change as a function of coverage. To introduce the dynamics of the system one writes down a model Hamiltonian which, for the simplest system of a one-component adsorbate with one adsorption site per unit cell, is... 

Arab, A.S., Yocum, G.T., Kalish, H. et al (2004) Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood 104, 1217-1223. 

Apart from attaching MRI contrast agents to the cell surface, cell labeling may also be achieved by using cell uptake processes. Endocytosis, whether receptor mediated or not, and phagocytosis are mechanisms that maybe considered and that could lead to accumulation of a magnetic label inside the cell. Provided the... 

I 6 van den Bos EJ, Wagner A, Mahrholdt H, et al, Improved efficacy of stem cell labeling for magnetic resonance imaging studies by the use of cationic liposomes. Cell Transpl 2003 12 743-756. 

GLYCO-MNPs AS NANOPROBES FOR LABELING CELLS AND MAGNETIC RESONANCE IMAGING AGENTS... 

Jendelova P, Herynek V, Urdzikova L, et al. Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparitcles in rat brain and spinal cord. J Neurosci Res 2004 76 232-43. 

Wilhelm C, Gazeau F (2008) Universal cell labelling with anionic magnetic nanoparticles. Biomaterials 29 3161-3174... 

To evaluate the association of siRNA transfection complexes with cells, and their internalization into cells, prepare the transfection complexes with rhodamine-labeled GFP-siRNA (siRNA-Rho), according to steps of Subheading 3.6, and perform transfection of the cells as described in Subheading 3.7. Alternatively, the rhodamine-labeled magnetic nanoparticles synthesized according to step 8 from Subheading 3.1 of the protocol can be used. 

Make sure to have enough cells. Cell density (50-100) x 10 cells per ml is sufficient to perform FACS analysis. Perform FACS analysis as quickly as possible to avoid cell aggregation and aging in the FACS buffer. Cells are magnetically labeled post-magnetofection due to association with magnetic transfection complexes vortex cells before FACS analysis. 

Ruthenium (II) tris(bipyridyl) (Figure 9-17, B) undergoes an electrochemiluminescent reaction (620 nm) with tripropylamine at an electrode surface, and this chelate is now used as a label in competitive and sandwich electrochemiluminescence immunoassays. Using this label, various assays have been developed in a flow cell using magnetic beads as the solid phase. Beads are captured at the electrode surface, and an unbound label is washed out of the cell by a wash buffer. Label bound to the bead undergoes an electrochemiluminescent reaction, and the light emission is measured by an adjacent photomultiplier tube. ... 

Cellular labeling/cell separation Cell labeling with MNPs is a method for in vivo cell separation, as the labeled cells can be detected by magnetic resonance imaging (MRI) [187, 188]. 

In magnetic cell sorting (MACS), cells labeled with primary antibody are incubated with a ferrous conjugated second antibody. Labeled cells are separated by decanting the cell suspension in a magnetic field. Pizzonia et al. [101] and Bacskai and co-authors [102] introduced MACS for immunoselection of murine distal tubule cells. Baer et al. [103] used MACS for im-munodissection of human proximal and distal tubule... 

In a second example, Inglis and coworkers developed a magnetic cell sorter based on an array of microfabricated magnetic stripes [3]. In this device, non-labeled cells and cells labeled with magnetic beads are mixed together and injected into the channel. The direction of the... 

Sundstrom IB, Mao H, Santoianni R et al. Magnetic resonance imaging of activated proliferating rhesus macaque T cells labeled with superparamagnetic monocrystaUine iron oxide nanoparticles. I Acquir Immune Befic Syndr 2004 35 9 21. 

Lu, C.-W. et al (2007) Bifimctional magnetic silica nanoparticles for highly efficient human stem cell labeling. Nano Lett, 7 (1), 149-154. 

Figure 2. ENDOR (a) and Special TRIPLE spectra (b) of D in single crystals of %-labeled RC s. For the shown orientations, the field is parallel to one of the three symmetry axes of the crystal, and all four RC s in the unit cell are magnetically equivalent. Line positions in (a) correspond to the high-frequency ENDOR transition (see eq. (2b)) = 1.46 MHz the indicated assignment to and...

Tseng, C. L., I, L. Shih. et al. 2010. Gadolinium hexanedione nanoparticles for stem cell labeling and tracking via magnetic resonance imaging. Biomaterials 31 5427-5435. 

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