Collagen cartilage tissue engineering

Martini, J., Tonsing, K., Dickob, M., Schade, R., Liefeith, K. and Anselmetti, D. (2006) 2-photon laser scanning microscopy on native human cartilage and collagen-membranes for tissue engineering. Proc. SPIE 6089, 274-282. 

Yan LP, Wang YJ, Wu G et al (2010) Genipin-cross-linked collagen/chitosan biomimetic scaffolds for articular cartilage tissue engineering applications. J Biomed Mater Res 95A 465 75... 

Shi, D., Cai, D. Zhou, C. (2005a) [Fabrication and properties of a composite chitosan/ type II collagen scaffold for tissue engineering cartilage]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, 19, 278-82. 

Wu and co-workers [13] incorporated copolymer poly(3HB-co-3HV) with calcium silicate(s) (CS) to increase the hydrophilicity of the copolymer in order to enhance cell adhesion on scaffolds used for cartilage tissue engineering. Interactions between poly(3HB-co-3HV)/CS composite scaffolds and chondrocytes in vitro and the formation of neocartilage were evaluated after the implantation of scaffolds into rabbits. It was found that the adhesion of chondrocytes onto the scaffolds and cell proliferation improved with the addition of CS. Enhanced penetration of chondrocytes into the scaffolds was observed with the increase in hydrophilicity of the poly(3HB-co-3HV)/CS composite scaffolds. A higher amount of collagen and glycosaminoglycan were detected in the composite scaffold compared with pure poly(3HB-co-3HV), indicating that poly(3HB-co-3HV)/CS composite scaffolds stimulated the extracellular matrix synthesis of chondrocytes. 

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