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Carbohydrates analyze

Fig.4.20. Diagram of the carbohydrate analyzer which uses heat-treatment detection. (From ref. 46 with permission of the author.)... Fig.4.20. Diagram of the carbohydrate analyzer which uses heat-treatment detection. (From ref. 46 with permission of the author.)...
MINIMUM CONCENTRATIONS OF SUGARS WHICH MAY BE DETECTED WITH THE MARK III CARBOHYDRATE ANALYZER... [Pg.133]

Nearly any type of continuous eluent gradient can be generated by connecting two or more chambers containing solutions of different properties to a common mixing chamber (Fig. 4). (See also the description of UV- and carbohydrate analyzers.)... [Pg.9]

The UV- and carbohydrate analyzers use a sample injection valve that contains six ports, each pair of which is interconnected. In one orientation of the valve, a sample can be loaded into the sample loop, which becomes a part of the eluent line when the ports are reoriented (by turning the valve handle) (Fig. 5). Valves that allow automated sample introduction at pressures up to 5000 psi without interrupting the eluent flow have been developed and are now available commercially (S2). [Pg.10]

All the systems discussed here use conventional strip chart recorders for recording the photometer or colorimeter output, and the resulting record is a conventional histogram in which the absorbance of the eluate or eluate-reagent reaction mixture is recorded as a function of time. In addition, some prototype systems of the UV- and carbohydrate analyzers use on-line computers for data storage and processing (Cl, S7). [Pg.11]

Fig. 12. The Mark II carbohydrate analyzer. From Scott (S5) with permission. Fig. 12. The Mark II carbohydrate analyzer. From Scott (S5) with permission.
Fig. 13. Typical chromatograms from the carbohydrate analyzer showing the difference between urine and blood serum and the identification of some of the chromatographic peaks. Sample sizes sugar reference compounds top), 0.62 pM except 125 pM mehbiose and... [Pg.21]

The carbohydrate analyzer has separated as many as 48 chromatographic peaks from a single body fluid sample however, chromatograms from urine samples of normal subjects have 30-40 peaks (Fig. 13) (S5). The carbohydrate analyzer is sensitive to a few micrograms of each individual carbohydrate. [Pg.25]

So far, the tentative chromatographic method has been used to make most of the identifications of the ninhydrin-positive and organic acid components, especially for urine constituents. This simply requires that the unknown peak has the same elution volume as a known reference compound. A significant effort has been made to provide more definite identifications for the components separated by the UV- and carbohydrate analyzers. To date, this has included over 70 UV-absorbing compounds and 18 carbohydrates, some of which are listed in Tables 1-3 (B2, M2). Tentative identification of many more compounds has been made in all four systems, and, hopefully, the efforts in confirmative identification will continue. [Pg.27]

Some op the Compounds Separated from Urine op Normal Subjects by THE Carbohydrate Analyzer and Identified by Gas Chromatography"... [Pg.32]

The carbohydrate analyzer has shown that there are considerable differences in excretion patterns of carbohydrates in disease. Many carbohydrates are excreted in excess in renal glycosuria and diabetes mellitus (Yl). Other abnormalities, such as pancreatic insufiSciency and lactose deficiency, show several carbohydrate excretion abnormalities. The presence of large amounts of xylulose and other sugars during ingestion of xylose indicates that the xylose tolerance test may not be a true measurement of absorption since that sugar apparently also metabolizes (Yl). [Pg.36]

The orcinol-sulfuric acid method, in which the recommended reagent is a solution of orcinol (0.1%, m/v) in diluted sulfuric acid (70%, v/v), was the first to be adapted for use in an automated carbohydrate analyzer. After mixing with the reagent, the column effluent is heated at 95°C for 15 min. Absorbance is usually measured at 420-425 nm howevei measurement at two wavelengths (420 and 510 nm) has been recommended where deoxy sugars or uronic acids are present. [Pg.429]

FIGURE 9. Chromatogram of carbohydrates in normal human urine (bottom) and in urine of two patients with galactosemia using carbohydrate analyzer developed at Oak Ridge. [Pg.138]

The earliest examples of analytical methods based on chemical kinetics, which date from the late nineteenth century, took advantage of the catalytic activity of enzymes. Typically, the enzyme was added to a solution containing a suitable substrate, and the reaction between the two was monitored for a fixed time. The enzyme s activity was determined by measuring the amount of substrate that had reacted. Enzymes also were used in procedures for the quantitative analysis of hydrogen peroxide and carbohydrates. The application of catalytic reactions continued in the first half of the twentieth century, and developments included the use of nonenzymatic catalysts, noncatalytic reactions, and differences in reaction rates when analyzing samples with several analytes. [Pg.623]

The data sets presented by Ambrose and Norr (1993) and Tiezsen and Fagre (1993) record analyses of rodents fed on diets in whose dietary components have been analyzed. Thus D and F are given for the diets, (generally for the protein, carbohydrate and lipid components sometimes in more detail), and have been varied so as to relate to the corresponding change in B. Measurements were made on animals thought to have reached an overall steady state. [Pg.219]

F.a.b.-m.s. has contributed to a detailed analysis of the high-mannose carbohydrate chains of prostaglandin endoperoxide synthase from sheep. Oligosaccharides released by hydrazinolysis were analyzed after N-re-acetylation and reduction. The f.a.b. data defined the compositions of the oligosaccharides, and showed that hydrazinolysis had effected a partial fission of the chitobiose core. [Pg.63]

Several groups have analyzed a variety of standard oligosaccharides and glycosides by using m.s.-m.s. techniques " (for a description of m.s.-m.s., see Ref. 131). None of this work gave convincing evidence that m.s.-m.s. is needed, or, indeed, is able, to solve carbohydrate problems, and for this reason, a discussion of m.s.-m.s. is not included herein. [Pg.70]

Crude chloroform-methanol-water (30 60 8, v/v) extracts of immunostainedTLC bands were analyzed without further purification by nanoelectrospray low-energy mass spectrometry. The authors showed that this effective PLC/MS-joined procedure offers a wide range of applications for any carbohydrate-binding agents such as bacterial toxins, plant lectins, and others. Phenyl-boronic acid (PBA) immobilized on stationary support phases can be put to similar applications. This technology, named boronate affinity chromatography (BAC), consists of a chemical reaction of 1,2- and 1,3-diols with the bonded-phase PBA to form a stable... [Pg.209]

The withdrawn hquid-phase samples were analyzed with an HPLC (Biorad Aminex HPX-87C carbohydrate coluttm. 1.2 ttiM CaS04 in deionized water was used as a mobile phase, since calcium ions improve the resolution of lactobionic acid [17]). Dissolved metals were analysed by Direct Current Plasma (DCP). The catalysts were characterized by (nitrogen adsorption BET, XPS surface analysis, SEM-EDXA, hydrogen TPD and particle size analysis). [Pg.105]


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See also in sourсe #XX -- [ Pg.25 ]




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